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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF572 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CXCL11/I-TAC Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CXCL11/I-TAC in ELISAs and Western blots. In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant human CXCL11, recombinant mouse (rm) BLC, rmCRG-2, rmGCP-2, rmMIG, rmPF4, and rmSDF-1 alpha is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Complementary Activity of ETV5, RBPJ, and TCF3 Drives Formative Transition from Naive Pluripotency.
Sensing and alarm function of resident memory CD8⁺ T cells.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Kalkan T, Bornelöv S, Mulas C, Diamanti E, Lohoff T, Ralser M, Middelkamp S, Lombard P, Nichols J, Smith A
Cell stem cell 2019 May 2;24(5):785-801.e7
Cell stem cell 2019 May 2;24(5):785-801.e7
Sensing and alarm function of resident memory CD8⁺ T cells.
Schenkel JM, Fraser KA, Vezys V, Masopust D
Nature immunology 2013 May;14(5):509-13
Nature immunology 2013 May;14(5):509-13
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Chemotaxis Induced by CXCL11/I-TAC and Neutralization by Mouse CXCL11/I-TAC Antibody. Recombinant Mouse CXCL11/I-TAC (Catalog # 572-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL11/ I-TAC (200 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL11/I-TAC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF572). The ND50 is typically 2-12 µg/mL.
