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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB572 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CXCL11/I-TAC Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CXCL11/I-TAC in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse CXC1, 2, 6, 9, 10, CXCL12/SDF-1 alpha , 13, 14, recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, CXCL12/SDF-1 alpha , CXCL12/SDF-1 beta , 13, 14, 15, recombinant rat CXCL1, 2, CXCL3/CINC2 alpha , CXCL3/CINC-2 beta , 5, or recombinant porcine CXCL8 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q9JHH5- Isotype
- IgG
- Antibody clone number
- 131327
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Rho GTPases control ciliary epithelium cells proliferation and progenitor profile induction in vivo.
The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
Del Debbio CB, Santos MF, Yan CY, Ahmad I, Hamassaki DE
Investigative ophthalmology & visual science 2014 Apr 21;55(4):2631-41
Investigative ophthalmology & visual science 2014 Apr 21;55(4):2631-41
The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
Fujita H, Asahina A, Komine M, Tamaki K
Cellular immunology 2007 Feb;245(2):70-9
Cellular immunology 2007 Feb;245(2):70-9
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Chemotaxis Induced by CXCL11/I-TAC and Neutral-ization by Mouse CXCL11/ I-TAC Antibody. Recombinant Mouse CXCL11/ I-TAC (Catalog # 572-MC) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL11/ I-TAC (50 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL11/I-TAC Monoclonal Antibody (Catalog # MAB572). The ND50 is typically 0.4-2.0 µg/mL.
