500-P132-100UG

antibody from Invitrogen Antibodies

Targeting: CXCL11 b-R1, H174, I-TAC, IP-9, SCYB11, SCYB9B

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P132-100UG

Provider

Invitrogen Antibodies

Product name

CXCL11 Polyclonal Antibody, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: FPMFKRGRCL CIGPGVKAVK VADIEKASIM YPSNNCDKIE VIITLKENKG QRCLNPKSKQ ARLIIKKVER KNF. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human I-TAC (CXCL11). Anti-Human I-TAC (CXCL11)-specific antibody was purified by affinity chromatography employing an immobilized Human I-TAC (CXCL11) matrix. Sandwich ELISA: To detect Human I-TAC (CXCL11) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human I-TAC (CXCL11) (500-P132Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human I-TAC (CXCL11). Western Blot: To detect Human I-TAC (CXCL11) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human I-TAC (CXCL11) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Reactivity

Human

Host

Rabbit

Vial size

100 µg

Concentration

0.1-1.0 mg/mL

Storage

-20°C

References

Submitted references

Human mast cell activation with virus-associated stimuli leads to the selective chemotaxis of natural killer cells by a CXCL8-dependent mechanism.

Burke SM, Issekutz TB, Mohan K, Lee PW, Shmulevitz M, Marshall JS
Blood 2008 Jun 15;111(12):5467-76

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Polarized localization of epithelial CXCL11 in chronic obstructive pulmonary disease and mechanisms of T cell egression.

Porter JC, Falzon M, Hall A
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 1;180(3):1866-77

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Elevated serum levels of the CXCR3 chemokine ITAC are associated with the development of transplant coronary artery disease.

Kao J, Kobashigawa J, Fishbein MC, MacLellan WR, Burdick MD, Belperio JA, Strieter RM
Circulation 2003 Apr 22;107(15):1958-61

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human I-TAC (CXCL11) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CXCL11 Polyclonal Antibody (Product # 500-P132-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CXCL11 Polyclonal Antibody, Biotin (Product # 500-P132BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human I-TAC (CXCL11).