500-P132BT-1MG

antibody from Invitrogen Antibodies

Targeting: CXCL11 b-R1, H174, I-TAC, IP-9, SCYB11, SCYB9B

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P132BT-1MG

Provider

Invitrogen Antibodies

Product name

CXCL11 Polyclonal Antibody, Biotin, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: FPMFKRGRCL CIGPGVKAVK VADIEKASIM YPSNNCDKIE VIITLKENKG QRCLNPKSKQ ARLIIKKVER KNF. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human I-TAC (CXCL11). Anti-Human I-TAC (CXCL11)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human I-TAC (CXCL11) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human I-TAC (CXCL11) (500-P132) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human I-TAC (CXCL11). Western Blot: To detect hI-TAC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hI-TAC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P132BT-1MG will be provided as 2 x 500 µg

Reactivity

Human

Host

Rabbit

Conjugate

Biotin

Vial size

2 x 500 µg

Concentration

0.1-1.0 mg/mL

Storage

-20°C

References

Submitted references

The Positively Charged COOH-terminal Glycosaminoglycan-binding CXCL9(74-103) Peptide Inhibits CXCL8-induced Neutrophil Extravasation and Monosodium Urate Crystal-induced Gout in Mice.

Vanheule V, Janssens R, Boff D, Kitic N, Berghmans N, Ronsse I, Kungl AJ, Amaral FA, Teixeira MM, Van Damme J, Proost P, Mortier A
The Journal of biological chemistry 2015 Aug 28;290(35):21292-304

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Citrullination of CXCL10 and CXCL11 by peptidylarginine deiminase: a naturally occurring posttranslational modification of chemokines and new dimension of immunoregulation.

Loos T, Mortier A, Gouwy M, Ronsse I, Put W, Lenaerts JP, Van Damme J, Proost P
Blood 2008 Oct 1;112(7):2648-56

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Human mast cell activation with virus-associated stimuli leads to the selective chemotaxis of natural killer cells by a CXCL8-dependent mechanism.

Burke SM, Issekutz TB, Mohan K, Lee PW, Shmulevitz M, Marshall JS
Blood 2008 Jun 15;111(12):5467-76

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect hI-TAC by Western Blot analysis CXCL11 Polyclonal Antibody, Biotin (Product # 500-P132BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hI-TAC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect hI-TAC by Western Blot analysis CXCL11 Polyclonal Antibody, Biotin (Product # 500-P132BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hI-TAC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Conjugate

Biotin

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human I-TAC (CXCL11) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of CXCL11 Polyclonal Antibody, Biotin (Product # 500-P132BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech CXCL11 Polyclonal Antibody (Product # 500-P132-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human I-TAC (CXCL11).

Conjugate

Biotin