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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunohistochemistry [1]
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- Product number
- LS-C101181 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-C101181, RRID:AB_2161849
- Product name
- Syndapin I / PACSIN1 Antibody (aa8-38) LS-C101181
- Antibody type
- Polyclonal
- Description
- Protein A purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of lysates from human brain, mouse brain and rat brain tissue lysate (from left to right), using PACSIN1 Antibody (G23). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of lysates from Daudi cell line, human heart and pancreas tissue lysate(from left to right), using PACSIN1 Antibody (G23). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of anti-PACSIN1 antibody in HL-60 cell line lysate. PACSIN1 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of anti-PACSIN1 antibody in mouse brain tissue lysate. PACSIN1 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of PACSIN1 (arrow) using rabbit polyclonal PACSIN1 Antibody (G23). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PACSIN1 gene.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
