AP8087a

antibody from Abcepta

Targeting: PACSIN1 SDPI

Antibody data

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Validation data

Product number: AP8087a - Provider product page
Provider: Abcepta
Proper citation: Abgent Cat#AP8087a, RRID:AB_2161841
Product name: PACSIN1 Antibody (N-term)
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Purified Rabbit Polyclonal Antibody (Pab)
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 400 µl
Concentration: 2 mg/ml
Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

PACSIN1 protein structure - AP8087a shown in red.

Supportive validation

Submitted by: Abcepta (provider)
Main image
Experimental details: "Western blot analysis of lysates from human brain, mouse brain and rat brain tissue lysate (from left to right), using PACSIN1 Antibody (G23)(Cat. #AP8087a). AP8087a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane."
Primary Ab dilution: 1:1000

Supportive validation

Submitted by: Abcepta (provider)
Main image
Experimental details: "Western blot analysis of lysates from Daudi cell line, human heart and pancreas tissue lysate(from left to right), using PACSIN1 Antibody (G23)(Cat. #AP8087a). AP8087a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane."
Primary Ab dilution: 1:1000

Supportive validation

Submitted by: Abcepta (provider)
Main image
Experimental details: Western blot analysis of anti-PACSIN1 Pab (Cat. #AP8087a) in HL-60 cell line lysate. PACSIN1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Primary Ab dilution: 1:1000

Supportive validation

Submitted by: Abcepta (provider)
Main image
Experimental details: Western blot analysis of PACSIN1 (arrow) using rabbit polyclonal PACSIN1 Antibody (G23) (Cat. #AP8087a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PACSIN1 gene.
Primary Ab dilution: 1:1000

Supportive validation

Submitted by: Abcepta (provider)
Main image
Experimental details: Western blot analysis of anti-PACSIN1 Pab (Cat. #AP8087a) in mouse brain tissue lysate . PACSIN1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Primary Ab dilution: 1:1000