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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-28852 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PACSIN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: Molt-4, mouse brain, rat brain. Predicted reactivity: Mouse (95%), Rat (95%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.7 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PACSIN1 using 20 µg of mouse brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PACSIN1 polyclonal antibody (Product # PA5-28852) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PACSIN1 was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a PACSIN1 Polyclonal Antibody (Product # PA5-28852) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PACSIN1 Polyclonal Antibody (Product # PA5-28852). Sample (30 µg of whole cell lysate). A: MOLT4SDS PAGE. Z. 7.5% SDS PAGE. PACSIN1 Polyclonal Antibody (Product # PA5-28852) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PACSIN1 was performed in DIV14 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: PACSIN1 Polyclonal Antibody (Product # PA5-28852) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PACSIN1 Polyclonal Antibody detects PACSIN1 protein at cytoplasm on MCF7 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded MCF7 xenograft. PACSIN1 Polyclonal Antibody (Product # PA5-28852) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
