LS-B10800

antibody from LSBio

Targeting: RAB6A RAB6

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B10800

Provider

LSBio

Product name

IHC-plus™ RAB6A / RAB6 Antibody (aa1-208) LS-B10800

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate). A: H1299. 12% SDS PAGE. RAB6 antibody diluted at 1:1000.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (50 ug of whole cell lysate). A: Mouse brain lysate/extract. 12% SDS PAGE. RAB6 antibody diluted at 1:1000.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (50 ug of whole cell lysate). A: Rat brain lysate/extract. 12% SDS PAGE. RAB6 antibody diluted at 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa using RAB6A antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa using RAB6A antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa using RAB6A antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa using RAB6A antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-RAB6A / RAB6 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.