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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- PA5-37782 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-ACK1 (Tyr284) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is HepG2 cells; suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is A549 cells.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from HepG2 cells treated with EGF using ACK1 (pTyr284) polyclonal antibody (Product # PA5-37782) (left) or the same antibody preincubated with a blocking peptide (right).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of methanol-fixed A549 cells using ACK1 (pTyr284) polyclonal antibody (Product # PA5-37782).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of methanol-fixed A549 cells using ACK1 (pTyr284) polyclonal antibody (Product # PA5-37782).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Phospho-ACK1 (Tyr284) in methanol-fixed A549 cells, using Phospho-ACK1 (Tyr284) Polyclonal Antibody (Product # PA5-37782).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-ACK1 (Tyr284) in paraffin-embedded Human breast carcinoma tissue using Phospho-ACK1 (Tyr284) Polyclonal Antibody (Product # PA5-37782) (left) or the same antibody preincubated with blocking peptide (right).
