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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [5]
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- Product number
- TA506153 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA506153, RRID:AB_2623645
- Product name
- DNM1L mouse monoclonal antibody, clone OTI4F6 (formerly 4F6)
- Antibody type
- Monoclonal
- Description
- DNM1L mouse monoclonal antibody, clone OTI4F6 (formerly 4F6)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- DNM1L
- Isotype
- IgG
- Antibody clone number
- OTI4F6
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Reactive oxygen species regulatory mechanisms associated with rapid response of MC3T3-E1 cells for vibration stress.
Zhang L, Gan X, Zhu Z, Yang Y, He Y, Yu H
Biochemical and biophysical research communications 2016 Feb 12;470(3):510-515
Biochemical and biophysical research communications 2016 Feb 12;470(3):510-515
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNM1L monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNM1L (RC221708, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNM1L.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 7 Human tissue by using anti-DNM1L monoclonal antibody ( 1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon) at 1:200 dilution.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506153)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506153)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506153)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506153)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506153)
- Validation comment
- IHC
