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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00010059-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00010059-M01, RRID:AB_437096
- Product name
- DNM1L monoclonal antibody (M01), clone 3B5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant DNM1L.
- Antigen sequence
MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQS
SGKSSVLESLVGRDLLPRGTGIVTRRPLILQLVHV
SQEDKRKTTGEENGVEAEEWGKFLHTKNKLYTDFD
EIRQEIENETERISGNNKGVSPEPIHLKIFSPNVV
NLTLVDLPGMTKVPVGDQPKDIELQIRELILRFIS
NPNSIILAVTAANTDMATSEALKISREVDPDGRRT
LAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVV
NRSQLDINNKKSVTDSIRDEYAFLQKKYPSLANRN
GTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQY
QSLLNSYGEPVDDKSATLLQLITKFATEYCNTIEG
TAKYIETSELCGGARICYIFHETFGRTLESVDPLG
GLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQ
IKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELL
RFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIE
LAYINTKHPDFADACGLMNNNIEEQRRNRLARELP
SAVSRDKLIQDSRRETKNVASGGGGVGDGVQEPTT
GNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGH
AVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFL
IVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLY
KSSLLDDLLTESEDMAQRRKEAADMLKALQGASQI
IAEIRETHLW- Isotype
- IgG
- Antibody clone number
- 3B5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Hyperinsulinemia induces insulin resistance in dorsal root ganglion neurons.
Mitochondrial biogenesis and fission in axons in cell culture and animal models of diabetic neuropathy.
Diabetes regulates mitochondrial biogenesis and fission in mouse neurons.
SUMOylation of the mitochondrial fission protein Drp1 occurs at multiple nonconsensus sites within the B domain and is linked to its activity cycle.
Kim B, McLean LL, Philip SS, Feldman EL
Endocrinology 2011 Oct;152(10):3638-47
Endocrinology 2011 Oct;152(10):3638-47
Mitochondrial biogenesis and fission in axons in cell culture and animal models of diabetic neuropathy.
Vincent AM, Edwards JL, McLean LL, Hong Y, Cerri F, Lopez I, Quattrini A, Feldman EL
Acta neuropathologica 2010 Oct;120(4):477-89
Acta neuropathologica 2010 Oct;120(4):477-89
Diabetes regulates mitochondrial biogenesis and fission in mouse neurons.
Edwards JL, Quattrini A, Lentz SI, Figueroa-Romero C, Cerri F, Backus C, Hong Y, Feldman EL
Diabetologia 2010 Jan;53(1):160-9
Diabetologia 2010 Jan;53(1):160-9
SUMOylation of the mitochondrial fission protein Drp1 occurs at multiple nonconsensus sites within the B domain and is linked to its activity cycle.
Figueroa-Romero C, Iñiguez-Lluhí JA, Stadler J, Chang CR, Arnoult D, Keller PJ, Hong Y, Blackstone C, Feldman EL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Nov;23(11):3917-27
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Nov;23(11):3917-27
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- DNM1L monoclonal antibody (M01), clone 3B5 Western Blot analysis of DNM1L expression in K-562 ( Cat # L009V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of DNM1L expression in transfected 293T cell line by DNM1L monoclonal antibody (M01), clone 3B5.Lane 1: DNM1L transfected lysate(79 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged DNM1L is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to DNM1L on formalin-fixed paraffin-embedded human leiomyosarcoma tissue. [antibody concentration 1 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
