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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00010059-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010059-M01, RRID:AB_538285
- Product name
- Mouse Monoclonal DRP1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. DNM1L - dynamin 1-like
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Mitochondrial Reactive Oxygen Species in Lipotoxic Hearts Induce Post-Translational Modifications of AKAP121, DRP1, and OPA1 That Promote Mitochondrial Fission.
Increase in proteins involved in mitochondrial fission, mitophagy, proteolysis and antioxidant response in type I endometrial cancer as an adaptive response to respiratory complex I deficiency.
Dietary supplementation with acetyl-l-carnitine counteracts age-related alterations of mitochondrial biogenesis, dynamics and antioxidant defenses in brain of old rats.
Hyperinsulinemia induces insulin resistance in dorsal root ganglion neurons.
Mitochondrial biogenesis and fission in axons in cell culture and animal models of diabetic neuropathy.
Diabetes regulates mitochondrial biogenesis and fission in mouse neurons.
SUMOylation of the mitochondrial fission protein Drp1 occurs at multiple nonconsensus sites within the B domain and is linked to its activity cycle.
Tsushima K, Bugger H, Wende AR, Soto J, Jenson GA, Tor AR, McGlauflin R, Kenny HC, Zhang Y, Souvenir R, Hu XX, Sloan CL, Pereira RO, Lira VA, Spitzer KW, Sharp TL, Shoghi KI, Sparagna GC, Rog-Zielinska EA, Kohl P, Khalimonchuk O, Schaffer JE, Abel ED
Circulation research 2018 Jan 5;122(1):58-73
Circulation research 2018 Jan 5;122(1):58-73
Increase in proteins involved in mitochondrial fission, mitophagy, proteolysis and antioxidant response in type I endometrial cancer as an adaptive response to respiratory complex I deficiency.
Cormio A, Musicco C, Gasparre G, Cormio G, Pesce V, Sardanelli AM, Gadaleta MN
Biochemical and biophysical research communications 2017 Sep 9;491(1):85-90
Biochemical and biophysical research communications 2017 Sep 9;491(1):85-90
Dietary supplementation with acetyl-l-carnitine counteracts age-related alterations of mitochondrial biogenesis, dynamics and antioxidant defenses in brain of old rats.
Nicassio L, Fracasso F, Sirago G, Musicco C, Picca A, Marzetti E, Calvani R, Cantatore P, Gadaleta MN, Pesce V
Experimental gerontology 2017 Nov;98:99-109
Experimental gerontology 2017 Nov;98:99-109
Hyperinsulinemia induces insulin resistance in dorsal root ganglion neurons.
Kim B, McLean LL, Philip SS, Feldman EL
Endocrinology 2011 Oct;152(10):3638-47
Endocrinology 2011 Oct;152(10):3638-47
Mitochondrial biogenesis and fission in axons in cell culture and animal models of diabetic neuropathy.
Vincent AM, Edwards JL, McLean LL, Hong Y, Cerri F, Lopez I, Quattrini A, Feldman EL
Acta neuropathologica 2010 Oct;120(4):477-89
Acta neuropathologica 2010 Oct;120(4):477-89
Diabetes regulates mitochondrial biogenesis and fission in mouse neurons.
Edwards JL, Quattrini A, Lentz SI, Figueroa-Romero C, Cerri F, Backus C, Hong Y, Feldman EL
Diabetologia 2010 Jan;53(1):160-9
Diabetologia 2010 Jan;53(1):160-9
SUMOylation of the mitochondrial fission protein Drp1 occurs at multiple nonconsensus sites within the B domain and is linked to its activity cycle.
Figueroa-Romero C, Iñiguez-Lluhí JA, Stadler J, Chang CR, Arnoult D, Keller PJ, Hong Y, Blackstone C, Feldman EL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Nov;23(11):3917-27
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Nov;23(11):3917-27
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DRP1 Antibody (3B5) [H00010059-M01] - Analysis of DNM1L expression in transfected 293T cell line by DNM1L monoclonal antibody (M01), clone 3B5.Lane 1: DNM1L transfected lysate(79 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DRP1 Antibody (3B5) [H00010059-M01] - DNM1L monoclonal antibody (M01), clone 3B5 Analysis of DNM1L expression in K-562.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: DRP1 Antibody (3B5) [H00010059-M01] - Detection limit for recombinant GST tagged DNM1L is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DRP1 Antibody (3B5) [H00010059-M01] - Analysis of monoclonal antibody to DNM1L on formalin-fixed paraffin-embedded human leiomyosarcoma tissue. Antibody concentration 1 ug/ml.
