A00556-2

antibody from Boster Biological Technology

Targeting: DNM1L DRP1, DVLP, DYMPLE, HDYNIV, VPS1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A00556-2

Provider

Boster Biological Technology

Product name

Anti-DRP1/DNM1L Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for DRP1/DNM1L detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study.

Zheng H, Liang G, Guan C, Liu L, Dong J, Zhao J, Tang M, Kong L
Nanomaterials (Basel, Switzerland) 2024 Apr 17;14(8)

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Buyang Huanwu Decoction alleviates cerebral ischemic injury through modulating caveolin-1-mediated mitochondrial quality control.

Xu Y, Chen B, Yi J, Tian F, Liu Y, Ouyang Y, Yuan C, Liu B
Frontiers in pharmacology 2023;14:1137609

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Shenlian extract decreases mitochondrial autophagy to regulate mitochondrial function in microvascular to alleviate coronary artery no-reflow.

Li JJ, Wang YJ, Wang CM, Li YJ, Yang Q, Cai WY, Chen Y, Zhu XX
Phytotherapy research : PTR 2023 May;37(5):1864-1882

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Neural stem cell secretome exerts a protective effect on damaged neuron mitochondria in Parkinson's disease model.

Ni W, Zhou J, Ling Y, Lu X, Niu D, Zeng Y, Qiu Y, Si Y, Wang J, Zhang W, Wang Z, Hu J
Brain research 2022 Sep 1;1790:147978

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Nicorandil, an ATP-sensitive potassium channel activation, attenuates myocardial injury in rats with ischemic cardiomyopathy.

Shaoqing L, Ting Z, Hao L, He Z, Wang Y, Ming Z
Medical molecular morphology 2022 Mar;55(1):41-46

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of DRP1/DNM1L using anti-DRP1/DNM1L antibody (A00556-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: rat brain tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRP1/DNM1L antigen affinity purified polyclonal antibody (Catalog # A00556-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DRP1/DNM1L at approximately 82KD. The expected band size for DRP1/DNM1L is at 82KD.

Additional image