12957-1-AP
antibody from Invitrogen Antibodies
Targeting: DNM1L
DRP1, DVLP, DYMPLE, HDYNIV, VPS1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- 12957-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DRP1 (C-terminal) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.16 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12957-1-AP ( DRP1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 12957-1-AP (DRP1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12957-1-AP (DRP1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were intracellularly stained with 0.2 µg Anti-Human DRP1 (C-terminal) (Product # 12957-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DRP1 (IP:12957-1-AP, 4ug; Detection:12957-1-AP 1:500) with mouse brain tissue lysate 3600ug.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DRP1 (C-terminal) (IP:12957-1-AP, 4 ug; Detection:12957-1-AP 1:1000) with mouse brain tissue lysate 2400 ug.