19142-1-AP

antibody from Proteintech Group

Targeting: MSTN GDF8

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

19142-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#19142-1-AP, RRID:AB_10638615

Product name

GDF8/Myostatin antibody

Antibody type

Polyclonal

Description

KD/KO validated GDF8/Myostatin antibody (Cat. #19142-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IHC, WB,ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

MSTN and TCF12 as Candidate Immunometabolic Signatures in Glioma-Associated Foam Cells: Insights from Integrated Multi-Omics Analysis.

Liu X, Song Z, Sun Z, Liu C, Kang X, Qiao H, Tu X, Li T, Fu Z, Wang Y
Current issues in molecular biology 2026 Mar 9;48(3)

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Sijunzi decoction alleviates cancer cachexia myatrophy by suppressing M2 polarization of tumor-associated macrophages via the MCT1/lactate/H3K18 lactylation pathway.

Nie D, Zhang Y, Jia R, Fang Z, Zhu G, Xu B, Guo T, Zhang H
Journal of ethnopharmacology 2026 Jun 12;364:121500

---

Asymmetrical paravertebral muscles fibrosis causes progression of adolescent idiopathic scoliosis via myostatin signalling in fibro-adipogenic progenitors.

Sun H, Huang Y, Zhou H, Chen H, Dai J, Zhao J, Huang X, Cai H, Shen J
Journal of orthopaedic translation 2026 Jan;56:101027

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MEGF8-mediated GDF8 phosphorylation drives TGF-β hyperactivation in osteoarthritis cartilage degeneration: Mechanism and targeted intervention.

Li W, Jia J, Xie R, Ni X, Cheng X, Kang S, Pan Y
Life sciences 2026 Jan 15;385:124139

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Exploring the mechanism of action of trastuzumab-induced cardiomyocyte atrophy based on the FN1/PI3K/AKT-mediated mTOR-independent signaling pathway.

Zhu M, Yang Y, Fang H, Chen R
Genomics 2025 Sep;117(5):111087

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Yolk extract-derived vitellogenin 2 ameliorates muscle atrophy in mice via the PI3K/AKT/mTOR pathway.

Li Y, Song P, Wang J, Tong Y, Hao X, Shi H, Ma P, Ji S, Yu X, Yan Y, Lu J, Luo X, Dong Y, Wang H
Food & function 2025 Sep 29;16(19):7663-7677

---

Epigenetic silencing of MSTN via m6A modification underlies the renoprotective effects of engineered MSC exosomes with RBM15 depletion in diabetic nephropathy.

Dong Q, Dong L, Zhu Y, Wang X, Yan X
Functional & integrative genomics 2025 Nov 17;25(1):244

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MEGF6 knockdown ameliorates lenvatinib-induced muscle differentiation suppression and enhances the antitumor effect of lenvatinib on hepatocellular carcinoma.

Qiang N, Ao J, Nakamura M, Katayama K, Zhang J, Kogure T, Ogawa K, Kanzaki H, Kojima R, Koroki K, Kobayashi K, Inoue M, Kanogawa N, Kiyono S, Nakagawa R, Kondo T, Ogasawara S, Nakamoto S, Muroyama R, Kato J, Kato N
Biochemical pharmacology 2025 May;235:116829

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Loss of Myostatin leads to low production of CH(4) by altering rumen microbiota and metabolome in cattle.

Hai C, Wang L, Wu D, Pei D, Yang Y, Liu X, Zhao Y, Bai C, Su G, Bao Z, Yang L, Li G
International journal of biological macromolecules 2025 Mar;294:139533

---

MyoMed205 Counteracts Titin Hyperphosphorylation and the Expression of Contraction-Regulating Proteins in a Rat Model of HFpEF.

Vahle B, Schauer A, Augstein A, Jarabo MP, Friedrich J, Barthel P, Männel A, Mangner N, Labeit S, Bowen TS, Linke A, Adams V
Journal of cachexia, sarcopenia and muscle 2025 Jun;16(3):e13843

---

Pharmacological HIF-PH Inhibition Suppresses Myoblast Differentiation Through Continued HIF-1α Stabilization.

Miki Y, Ochi A, Uedono H, Kakutani Y, Ichii M, Nagata Y, Mori K, Imanishi Y, Shoji T, Morioka T, Emoto M
International journal of molecular sciences 2025 Jun 5;26(11)

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Skeletal Muscle Alterations in Different Phenotypes of Heart Failure with Preserved Ejection Fraction.

Vahle B, Klädtke R, Schauer A, Bowen TS, Wisløff U, Linke A, Adams V
International journal of molecular sciences 2025 Jun 27;26(13)

---

Multi-therapeutic strategy targeting Akt-mTOR and FoxO1 pathway to counteract skeletal muscle atrophy consecutive to hypoxia.

Bensaid S, Fabre C, Yahya Rajaei A, Claeyssen C, Daussin FN, Cieniewski-Bernard C
American journal of physiology. Cell physiology 2025 Jun 1;328(6):C2057-C2069

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Muscle-homing peptides modified biomimetic curcumin nanoparticles ameliorate skeletal muscle dysfunction in aging mice.

Xie J, Huang Z, Gao N, Feng H, Wang B, Gao S, Tian H, Wu C, Liu C
Redox biology 2025 Jul;84:103679

---

Acetate Administration Ameliorates Streptozotocin-Induced Hyperglycemia and Adipose Tissue Loss.

Zhang C, Wang Z, Luo L, Liu X, Jia Z, Zhang Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 Jul 31;39(14):e70855

---

HouShiHeiSan attenuates sarcopenia in middle cerebral artery occlusion (MCAO) rats.

Qi H, Gao Y, Zhang Z, Zhang X, Tian D, Jiang Y, Zhang L, Zeng N, Yang R
Journal of ethnopharmacology 2025 Jan 30;337(Pt 3):118917

---

Endurance exercise remodels skeletal muscle by suppressing Ythdf1-mediated myostatin expression.

Huang X, Xu C, Zhang J, Wu W, Wang Z, Pang Q, Liu Z, Liu B
Cell death & disease 2025 Feb 13;16(1):96

---

Muscle homing peptide modified liposomes loaded with EGCG improved skeletal muscle dysfunction by inhibiting inflammation in aging mice.

Huang Z, Xie J, Gao N, Feng H, Wang B, Tian H, Wu C, Liu C
Materials today. Bio 2025 Dec;35:102265

---

Myostatin Exhibits an Evolutionarily Conserved Circadian Pattern in Skeletal Muscles.

Liu X, Song C, Xiong Y, Gu J, Wu L, Liu T, Chen X, Shu H, Dong Y, Shan T, Wang S, Zhu Y, Song T, Fu L, Lin Y, Liu C, Zheng R, Zhao X, Li H, Xu Y, Kuang S, Wang H, Guo B, Xu P, Jia Z
Journal of cachexia, sarcopenia and muscle 2025 Dec;16(6):e70130

---

Cytosine base editor-mediated high-efficiency myostatin editing in Hu sheep.

Wang Y, Liu WJ, Meng CH, Wang HL, Cui ZK, Zhang J, Zhang JL, Qian Y, Li YX, Cao SX
Functional & integrative genomics 2025 Aug 27;25(1):176

---

Collagen XII deficiency induces myogene expression in subcutaneous adipose tissues.

Kushige H, Saito F, Izu Y
Biochemical and biophysical research communications 2025 Aug 15;775:152124

---

Mechanistically based blood proteomic markers in the TGF-β pathway stratify risk of hepatocellular cancer in patients with cirrhosis.

Xiang X, Bhowmick K, Shetty K, Ohshiro K, Yang X, Wong LL, Yu H, Latham PS, Satapathy SK, Brennan C, Dima RJ, Chambwe N, Sharifova G, Cacaj F, John S, Crawford JM, Huang H, Dasarathy S, Krainer AR, He AR, Amdur RL, Mishra L
Genes & cancer 2024;15:1-14

---

In ovo betaine injection improves breast muscle growth in newly hatched goslings through FXR/IGF-2 pathway.

Ma S, Liu J, Zhao Y, Wang Y, Zhao R
Poultry science 2024 Oct;103(10):104075

---

Sulforaphane Promotes Proliferation of Porcine Granulosa Cells via the H3K27ac-Mediated GDF8-ALK5-ERK Pathway.

Zhou J, Fu C, Shen M, Tao J, Liu H
Journal of agricultural and food chemistry 2024 Oct 2;72(39):21635-21649

---

Modulation of Titin and Contraction-Regulating Proteins in a Rat Model of Heart Failure with Preserved Ejection Fraction: Limb vs. Diaphragmatic Muscle.

Vahle B, Heilmann L, Schauer A, Augstein A, Jarabo MP, Barthel P, Mangner N, Labeit S, Bowen TS, Linke A, Adams V
International journal of molecular sciences 2024 Jun 16;25(12)

---

Indoxyl sulphate-initiated activation of cardiac fibroblasts is modulated by aryl hydrocarbon receptor and nuclear factor-erythroid-2-related factor 2.

Barisione C, Verzola D, Garibaldi S, Altieri P, Furfaro AL, Nitti M, Pratesi G, Palombo D, Ameri P
Journal of cellular and molecular medicine 2024 Apr;28(7):e18192

---

Leucine Supplementation Improves Diastolic Function in HFpEF by HDAC4 Inhibition.

Alves PKN, Schauer A, Augstein A, Männel A, Barthel P, Joachim D, Friedrich J, Prieto ME, Moriscot AS, Linke A, Adams V
Cells 2023 Nov 2;12(21)

---

Blockade of Activin Receptor IIB Protects Arthritis Pathogenesis by Non-Amplification of Activin A-ACVR2B-NOX4 Axis Pathway.

Jeon J, Lee H, Jeon MS, Kim SJ, Choi C, Kim KW, Yang DJ, Lee S, Bae YS, Choi WI, Jung J, Eyun SI, Yang S
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 May;10(14):e2205161

---

Molecular Characterization of U6 Promoters from Orange-Spotted Grouper (Epinephelus coioides) and Its Application in DNA Vector-Based RNAi Technology.

Yan F, Xiao X, Long C, Tang L, Wang C, Zhang M, Zhang J, Lin H, Huang H, Zhang Y, Li S
Marine biotechnology (New York, N.Y.) 2023 Jun;25(3):388-402

---

Efficient and Specific Generation of MSTN-Edited Hu Sheep Using C-CRISPR.

Guo R, Wang H, Meng C, Gui H, Li Y, Chen F, Zhang C, Zhang H, Ding Q, Zhang J, Zhang J, Qian Y, Zhong J, Cao S
Genes 2023 Jun 2;14(6)

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The Effect of MSTN Mutation on Bile Acid Metabolism and Lipid Metabolism in Cattle.

Wu D, Wang S, Hai C, Wang L, Pei D, Bai C, Su G, Liu X, Zhao Y, Liu Z, Yang L, Li G
Metabolites 2023 Jul 11;13(7)

---

Capsaicin alleviates cisplatin-induced muscle loss and atrophy in vitro and in vivo.

Huang KC, Chiang YF, Huang TC, Chen HY, Lin PH, Ali M, Hsia SM
Journal of cachexia, sarcopenia and muscle 2023 Feb;14(1):182-197

---

Carboxyl-terminal modulator protein (CTMP) deficiency mitigates denervation-induced skeletal muscle atrophy.

Wang J, Tierney L, Wilson C, Phillips V, Goldman L, Mumaw C, Muang E, Walker CL
Biochemical and biophysical research communications 2023 Feb 12;644:155-161

---

Phosphate depletion in insulin-insensitive skeletal muscle drives AMPD activation and sarcopenia in chronic kidney disease.

Andres-Hernando A, Cicerchi C, Garcia GE, Orlicky DJ, Stenvinkel P, Johnson RJ, Lanaspa MA
iScience 2023 Apr 21;26(4):106355

---

Fish Protein Promotes Skeletal Muscle Hypertrophy via the Akt/mTOR Signaling Pathways.

Morisasa M, Yoshida E, Fujitani M, Kimura K, Uchida K, Kishida T, Mori T, Goto-Inoue N
Journal of nutritional science and vitaminology 2022;68(1):23-31

---

Creatine modulates cellular energy metabolism and protects against cancer cachexia-associated muscle wasting.

Wei L, Wang R, Lin K, Jin X, Li L, Wazir J, Pu W, Lian P, Lu R, Song S, Zhao Q, Li J, Wang H
Frontiers in pharmacology 2022;13:1086662

---

Expression patterns and correlation analyses of muscle-specific genes in the process of sheep myoblast differentiation.

Wang H, Dou M, Li J, Cao P, Li J, Guo T, Zhao D, Khan A, Li Y, Li B, Qin J, Du R
In vitro cellular & developmental biology. Animal 2022 Oct;58(9):798-809

---

A new therapeutic effect of fenofibrate in Duchenne muscular dystrophy: The promotion of myostatin degradation.

Sun Z, Xu D, Zhao L, Li X, Li S, Huang X, Li C, Sun L, Liu B, Jiang Z, Zhang L
British journal of pharmacology 2022 Mar;179(6):1237-1250

---

Differential Protein Metabolism and Regeneration in Gastrocnemius Muscles in High-fat Diet Fed Mice and Pre-hibernation Daurian Ground Squirrels: A Comparison between Pathological and Healthy Obesity.

Yan X, Niu Q, Gao X, Shen S, He N, Wang H, Fang R, Gao Y, Chang H
Zoological studies 2021;60:e6

---

Renal Ischemia/Reperfusion Early Induces Myostatin and PCSK9 Expression in Rat Kidneys and HK-2 Cells.

Barisione C, Verzola D, Garibaldi S, Ferrari PF, Garibotto G, Ameri P, Pane B, Spinella G, Pratesi G, Palombo D
International journal of molecular sciences 2021 Sep 13;22(18)

---

Vitellogenin 2 promotes muscle development and stimulates the browning of white fat.

Li Y, Sun X, Bai Y, Ji Y, Ren H, Yu X, Yan Y, He X, Dong Y, Zhang L, Luo X, Wang H
Aging 2021 Oct 5;13(19):22985-23003

---

Inhibition of heat shock protein (HSP) 90 reverses signal transducer and activator of transcription (STAT) 3-mediated muscle wasting in cancer cachexia mice.

Niu M, Song S, Su Z, Wei L, Li L, Pu W, Zhao C, Ding Y, Wang J, Cao W, Gao Q, Wang H
British journal of pharmacology 2021 Nov;178(22):4485-4500

---

Heat stress during late pregnancy of sows influences offspring longissimus dorsi muscle growth at weaning.

Li Y, Fan G, Liu Y, Yao W, Albrecht E, Zhao R, Yang X
Research in veterinary science 2021 May;136:336-342

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The Prophylactic Effects of Glutamine on Muscle Protein Synthesis and Degradation in Rats with Ethanol-Induced Liver Damage.

Xiao Q, Chen YH, Pratama SA, Chen YL, Shirakawa H, Peng HC, Yang SC
Nutrients 2021 Aug 14;13(8)

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Differential protein metabolism and regeneration in hypertrophic diaphragm and atrophic gastrocnemius muscles in hibernating Daurian ground squirrels.

Yan X, Gao X, Niu Q, Peng X, Zhang J, Ma X, Wei Y, Wang H, Gao Y, Chang H
Experimental physiology 2021 Apr;106(4):958-971

---

Myostatin in the Arterial Wall of Patients with End-Stage Renal Disease.

Esposito P, Verzola D, Porta E, Milanesi S, Grignano MA, Avella A, Gregorini M, Abelli M, Ticozzelli E, Rampino T, Garibotto G
Journal of atherosclerosis and thrombosis 2020 Oct 1;27(10):1039-1052

---

Skeletal Muscle Anti-Atrophic Effects of Leucine Involve Myostatin Inhibition.

Cruz A, Ferian A, Alves PKN, Silva WJ, Bento MR, Gasch A, Labeit S, Moriscot AS
DNA and cell biology 2020 Nov 2;

---

Effect of maternal nutrient restriction on skeletal muscle mass and associated molecular pathways in SGA and Non-SGA sheep fetuses.

Sandoval C, Lambo CA, Beason K, Dunlap KA, Satterfield MC
Domestic animal endocrinology 2020 Jul;72:106443

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Enhanced myostatin expression and signalling promote tubulointerstitial inflammation in diabetic nephropathy.

Verzola D, Milanesi S, Viazzi F, Ansaldo F, Saio M, Garibaldi S, Carta A, Costigliolo F, Salvidio G, Barisione C, Esposito P, Garibotto G, Picciotto D
Scientific reports 2020 Apr 14;10(1):6343

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Mitochondrial dysfunction and inhibition of myoblast differentiation in mice with high-fat-diet-induced pre-diabetes.

Xu D, Jiang Z, Sun Z, Wang L, Zhao G, Hassan HM, Fan S, Zhou W, Han S, Zhang L, Wang T
Journal of cellular physiology 2019 May;234(5):7510-7523

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Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes.

Lixin L, Juan W, Yun B, Jingwei L, Xiuju Y, Xiaomao L, Zhiwei Z, Xiaoyan H, Yanjun D, Hongquan L, Haidong W
Acta histochemica 2019 Jul;121(5):539-545

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Expression of diaphragmatic myostatin and correlation with apoptosis in rats with chronic obstructive pulmonary disease.

Zhou X, Yi D, Wu Y, Pei X, Yu H, Chen Y, Jiang Y, Li W
Experimental and therapeutic medicine 2018 Mar;15(3):2295-2300

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Molecular Cloning, Identification, and Expression Patterns of Myostatin Gene in Water Buffalo (Bubalus Bubalis).

Zhu P, Li H, Huang G, Cui J, Zhang R, Cui K, Yang S, Shi D
Animal biotechnology 2018 Jan 2;29(1):26-33

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Potential therapeutic interventions for chronic kidney disease-associated sarcopenia via indoxyl sulfate-induced mitochondrial dysfunction.

Enoki Y, Watanabe H, Arake R, Fujimura R, Ishiodori K, Imafuku T, Nishida K, Sugimoto R, Nagao S, Miyamura S, Ishima Y, Tanaka M, Matsushita K, Komaba H, Fukagawa M, Otagiri M, Maruyama T
Journal of cachexia, sarcopenia and muscle 2017 Oct;8(5):735-747

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Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1.

Enoki Y, Watanabe H, Arake R, Sugimoto R, Imafuku T, Tominaga Y, Ishima Y, Kotani S, Nakajima M, Tanaka M, Matsushita K, Fukagawa M, Otagiri M, Maruyama T
Scientific reports 2016 Aug 23;6:32084

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Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP.

Bi Y, Hua Z, Liu X, Hua W, Ren H, Xiao H, Zhang L, Li L, Wang Z, Laible G, Wang Y, Dong F, Zheng X
Scientific reports 2016 Aug 17;6:31729

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Myostatin, follistatin and activin type II receptors are highly expressed in adenomyosis.

Carrarelli P, Yen CF, Arcuri F, Funghi L, Tosti C, Wang TH, Huang JS, Petraglia F
Fertility and sterility 2015 Sep;104(3):744-52.e1

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse heart tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP( MSTN Antibody) at dilution of 1:600

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human heart using 19142-1-AP(MSTN antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The GDF8/Myostatin antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human GDF8/Myostatin. This antibody recognizes human,mouse,rat antigen. The GDF8/Myostatin antibody has been validated for the following applications: ELISA, IHC, WB analysis.

Appendix

19142-1-AP.pdf

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