Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
Reference
Comment
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- Product number
- bs-1288R - Provider product page
- Provider
- Bioss
- Proper citation
- Bioss Cat#bs-1288R, RRID:AB_10856184
- Product name
- GDF8/MSTN Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- KLH conjugated synthetic peptide derived from human GDF8
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1ug/ul
- Storage
- Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Submitted references Sulfiredoxin-1 protects against simulated ischaemia/reperfusion injury in cardiomyocyte by inhibiting PI3K/AKT-regulated mitochondrial apoptotic pathways.
Zhang J, He Z, Guo J, Li Z, Wang X, Yang C, Cui X
Bioscience reports 2016;36(2)
Bioscience reports 2016;36(2)
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Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Mouse skeletal muscle lysate probed with Anti GDF8/MSTN Polyclonal Antibody, Unconjugated (bs-1288R) at 1:200 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37°C. Predicted band 43kD. Observed band size: 43kD
- Sample type
- Mouse
- Other comments
- Muscle
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Generously provided by Bradley Elliott from University of Westminster as part of the Bioss Discovery Program. Lanes 1 and 2 are human muscle lysates and Lanes 3 and 4 are C2C12 cells. Rabbit Anti-GDF8/MSTN Polyclonal Antibody (bs-1288R) was used at a dilution of 1:1000, overnight at 4C.
- Sample type
- Human
- Other comments
- Muscle
Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded mouse small intestine labeled Anti-GDF8/MSTN Polyclonal Antibody, Unconjugated (bs-1288R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
- Sample type
- Mouse
- Other comments
- Intestine
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded rat heart tissue labeled Anti-GDF8/MSTN Polyclonal Antibody, Unconjugated (bs-1288R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
- Sample type
- Rat
- Other comments
- Heart