PA5-11936

antibody from Invitrogen Antibodies

Targeting: MSTN GDF8

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-11936 - Provider product page
Provider: Invitrogen Antibodies
Product name: Myostatin Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody is predicted to react with bovine, porcine and non-human primate based on sequence homology.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 200 µL
Concentration: 0.3 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

MSTN protein structure - PA5-11936 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 The effect of HY7715 treatment on age-induced sarcopenic factors in mice. ( A ) The plasma level of TNFalpha in mice was measured using a commercial colorimetric enzyme-linked immunosorbent assay (ELISA) kit. ( B ) Western blot analysis of myostatin and glyceraldehyde 3-phosphate dehydrogenase ( GAPDH ) in gastrocnemius muscle of the mice. The mRNA levels of muscle atrophy F-box gene ( Atrogin1 ) and muscle RING-finger protein 1 ( MuRf1 ) in gastrocnemius muscle. ( C ) The protein level of myostatin was quantified. The mRNA level of Atrogin1 and MuRf1 in gastrocnemius muscle ( D , E ) and soleus muscle ( F , G ) were normalized to the level of GAPDH mRNA and calculated as a relative-fold value. Statistical significance was determined using one-way ANOVA followed by Tukey's post hoc test (N = 5). Datasets denoted by different letters are significantly different; p < 0.05 (a > b > c). YM, young mice; OM, old mice; OM + C, creatine-treated old mice; OM + HY, HY7715-treated old mice.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Effect of MON on the expression of Atrogin1, MuRF1, and Myostatin in DEX-stimulated C2C12 myotubes. The expression of Atrogin1, MuRF1, and Myostatin was determined in the C2C12 myotubes by Western blotting ( A ). Relative expression of each protein was normalized to beta-actin ( B - D ). Each value is the mean +- SD of three independent experiments. ## p < 0.01, ### p < 0.001 vs. Nor; ** p < 0.01, *** p < 0.001 vs. DEX. Nor, normal group; DEX, dexamethasone-treated group; and MON, group-administered monotropein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 MON improved DEX-treated muscle atrophy in mice. The expression of MyHC, Atrogin1, MuRF1, and Myostatin was determined in gastrocnemius tissues of mice by Western blot ( A ). Relative expression of each protein was normalized to beta-actin ( B - E ). Each value is the mean +- SD of three independent experiments. # p < 0.05, ### p < 0.001 vs. Nor; * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. DEX. Nor, normal group; DEX, dexamethasone-treated group; and MON, monotropein-administrated group.