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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [3]
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- Product number
- PA5-11936 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Myostatin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, porcine and non-human primate based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Monotropein Improves Dexamethasone-Induced Muscle Atrophy via the AKT/mTOR/FOXO3a Signaling Pathways.
Lactobacillus plantarum HY7715 Ameliorates Sarcopenia by Improving Skeletal Muscle Mass and Function in Aged Balb/c Mice.
Myostatin knockdown and its effect on myogenic gene expression program in stably transfected goat myoblasts.
Wang P, Kang SY, Kim SJ, Park YK, Jung HW
Nutrients 2022 Apr 29;14(9)
Nutrients 2022 Apr 29;14(9)
Lactobacillus plantarum HY7715 Ameliorates Sarcopenia by Improving Skeletal Muscle Mass and Function in Aged Balb/c Mice.
Lee K, Kim J, Park SD, Shim JJ, Lee JL
International journal of molecular sciences 2021 Sep 16;22(18)
International journal of molecular sciences 2021 Sep 16;22(18)
Myostatin knockdown and its effect on myogenic gene expression program in stably transfected goat myoblasts.
Patel AK, Tripathi AK, Patel UA, Shah RK, Joshi CG
In vitro cellular & developmental biology. Animal 2014 Aug;50(7):587-96
In vitro cellular & developmental biology. Animal 2014 Aug;50(7):587-96
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a Myostatin polyclonal antibody (Product # PA5-11936) in mouse liver tissue lysate. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skeletal muscle tissue using a Myostatin polyclonal antibody (Product # PA5-11936), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 The effect of HY7715 treatment on age-induced sarcopenic factors in mice. ( A ) The plasma level of TNFalpha in mice was measured using a commercial colorimetric enzyme-linked immunosorbent assay (ELISA) kit. ( B ) Western blot analysis of myostatin and glyceraldehyde 3-phosphate dehydrogenase ( GAPDH ) in gastrocnemius muscle of the mice. The mRNA levels of muscle atrophy F-box gene ( Atrogin1 ) and muscle RING-finger protein 1 ( MuRf1 ) in gastrocnemius muscle. ( C ) The protein level of myostatin was quantified. The mRNA level of Atrogin1 and MuRf1 in gastrocnemius muscle ( D , E ) and soleus muscle ( F , G ) were normalized to the level of GAPDH mRNA and calculated as a relative-fold value. Statistical significance was determined using one-way ANOVA followed by Tukey's post hoc test (N = 5). Datasets denoted by different letters are significantly different; p < 0.05 (a > b > c). YM, young mice; OM, old mice; OM + C, creatine-treated old mice; OM + HY, HY7715-treated old mice.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Effect of MON on the expression of Atrogin1, MuRF1, and Myostatin in DEX-stimulated C2C12 myotubes. The expression of Atrogin1, MuRF1, and Myostatin was determined in the C2C12 myotubes by Western blotting ( A ). Relative expression of each protein was normalized to beta-actin ( B - D ). Each value is the mean +- SD of three independent experiments. ## p < 0.01, ### p < 0.001 vs. Nor; ** p < 0.01, *** p < 0.001 vs. DEX. Nor, normal group; DEX, dexamethasone-treated group; and MON, group-administered monotropein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 MON improved DEX-treated muscle atrophy in mice. The expression of MyHC, Atrogin1, MuRF1, and Myostatin was determined in gastrocnemius tissues of mice by Western blot ( A ). Relative expression of each protein was normalized to beta-actin ( B - E ). Each value is the mean +- SD of three independent experiments. # p < 0.05, ### p < 0.001 vs. Nor; * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. DEX. Nor, normal group; DEX, dexamethasone-treated group; and MON, monotropein-administrated group.
