PA5-79318

antibody from Invitrogen Antibodies

Targeting: GNAQ G-ALPHA-q, GAQ

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-79318

Provider

Invitrogen Antibodies

Product name

GNAQ Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: human placenta tissue, human Jurkat whole cell, rat brain tissue, mouse brain tissue. IHC: mouse testis tissue, rat ovary tissue, human prostatic cancer tissue. ICC/IF: A431 cell. Flow: A431 cell.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

References

Submitted references

Transcriptomic and metabolomic insights into the variety of sperm storage in oviduct of egg layers.

Yang G, Li S, Zhao Q, Chu J, Zhou B, Fan S, Shi F, Wei X, Hu X, Zheng X, Liu Z, Zhou X, Tao Y, Li S, Mou C
Poultry science 2021 Jun;100(6):101087

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Minute-scale persistence of a GPCR conformation state triggered by non-cognate G protein interactions primes signaling.

Gupte TM, Ritt M, Dysthe M, Malik RU, Sivaramakrishnan S
Nature communications 2019 Oct 23;10(1):4836

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of GNAQ using anti-GNAQ antibody (Product # PA5-79318). GNAQ was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-GNAQ antibody (Product # PA5-79318)overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of GNAQ using anti-GNAQ antibody (Product # PA5-79318). GNAQ was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-GNAQ antibody (Product # PA5-79318)overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GNAQ on paraffin-embedded rat ovary tissue. Sample was incubated with GNAQ polyclonal antibody (Product# PA5-79318).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GNAQ on paraffin-embedded human prostate cancer tissue. Sample was incubated with GNAQ polyclonal antibody (Product# PA5-79318).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GNAQ on paraffin-embedded mouse testis tissue. Sample was incubated with GNAQ polyclonal antibody (Product# PA5-79318).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry of GNAQ in A431 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with GNAQ Polyclonal Antibody (Product # PA5-79318) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry of GNAQ in A431 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with GNAQ Polyclonal Antibody (Product # PA5-79318) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Immunohistochemistry stained localization of ST6GAL1, GNAQ, ADFP, and PCNA in UVJ of egg layer breeders with high and low sperm storage capacity. (A-B) ST6GAL1 proteins were localized at epithelial cytoplasm in (A) high-SS and (B) low-SS hen UVJ. (C-D) The positive signals of GANQ antibody were localized at epithelial cell membranes in (C) high-SS and (D) low-SS hen UVJ. (E-F) The strong positive signals of ADFP antibody staining were converged on SST in (E) high-SS and (F) low-SS hen UVJ. (G-H) Positive PCNA signals appeared in all epithelial cells in (G) high-SS and (H) low-SS hen UVJ. (I) The negative control without incubating the primary antibody in immunohistochemistry. Abbreviations: SS, sperm storage; SST, sperm storage tubules; UVJ, uterovaginal junction; A-F and I, Bar, 100 mum. G and H, Bar, 200 mum.