M00898

antibody from Boster Biological Technology

Targeting: GNAQ G-ALPHA-q, GAQ

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M00898

Provider

Boster Biological Technology

Product name

Anti-GNAQ Antibody Picoband™ (monoclonal, 13H4)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for GNAQ detection. Tested with WB, IHC-P, FCM in Human;Monkey;Mouse;Rat.

Reactivity

Human, Mouse, Rat, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

13H4

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of GNAQ using anti-GNAQ antibody (M00898). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates. Lane 4: human A431 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: monkey COS-7 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat lung tissue lysates, Lane 10: mouse brain tissue lysates, Lane 11: mouse lung tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GNAQ antigen affinity purified monoclonal antibody (Catalog # M00898) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GNAQ at approximately 42KD. The expected band size for GNAQ is at 42KD.

Additional image