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- References [3]
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- Immunocytochemistry [1]
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- Product number
- MAB2570-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Nidogen-1/Entactin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Nidogen-1/Entactin in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Nidogen-2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AAH45606.1- Isotype
- IgG
- Antibody clone number
- 302117
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Endothelial cell-derived nidogen-1 inhibits migration of SK-BR-3 breast cancer cells.
Enhanced wound healing, kinase and stem cell marker expression in diabetic organ-cultured human corneas upon MMP-10 and cathepsin F gene silencing.
Normalization of wound healing and diabetic markers in organ cultured human diabetic corneas by adenoviral delivery of c-Met gene.
Ferraro DA, Patella F, Zanivan S, Donato C, Aceto N, Giannotta M, Dejana E, Diepenbruck M, Christofori G, Buess M
BMC cancer 2019 Apr 4;19(1):312
BMC cancer 2019 Apr 4;19(1):312
Enhanced wound healing, kinase and stem cell marker expression in diabetic organ-cultured human corneas upon MMP-10 and cathepsin F gene silencing.
Saghizadeh M, Epifantseva I, Hemmati DM, Ghiam CA, Brunken WJ, Ljubimov AV
Investigative ophthalmology & visual science 2013 Dec 17;54(13):8172-80
Investigative ophthalmology & visual science 2013 Dec 17;54(13):8172-80
Normalization of wound healing and diabetic markers in organ cultured human diabetic corneas by adenoviral delivery of c-Met gene.
Saghizadeh M, Kramerov AA, Yu FS, Castro MG, Ljubimov AV
Investigative ophthalmology & visual science 2010 Apr;51(4):1970-80
Investigative ophthalmology & visual science 2010 Apr;51(4):1970-80
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Nidogen-1/Entactin in Human Chondrocytes. Nidogen-1/Entactin was detected in immersion fixed human mesenchymal stem cells differentiated into chondrocytes using Mouse Anti-Human Nidogen-1/Entactin Monoclonal Antibody (Catalog # MAB2570) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
