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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- MA5-23911 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nidogen Monoclonal Antibody (302117)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, no cross-reactivity with recombinant human Nidogen-2 is observed. Reconstitute at 0.5 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 302117
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunocytochemistry analysis of Nidogen in immersion fixed human mesenchymal stem cells differentiated into chondrocytes. Samples were incubated in Nidogen monoclonal antibody (Product # MA5-23911) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow) and counterstained with DAPI (blue).
