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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-97016 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nidogen Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DLDTTDGLGK VYYREDLSPS ITQRAAECVH RGFPEISFQP SSAVVVTWES VAPYQGPSRD PDQKGKRNTF QAVLASSDSS SYAIFLYPED GLQFHTTFSK KENNQVPAVV AFSQGSVGFL WKSNGAYNIF ANDRESVENL AKSSNSGQQG VWVFEIGSPA TTNGVVPADV ILGTEDGAEY DDEDEDYDLA TTRLGLEDVG TTPFSYKALR RGGADTYSVP SVLSPRRAAT ERPLGPPTER TRSFQLAVET FHQQHPQVID VDEVEETGVV FSYNTDSRQT CANNRHQCSV H; Positive Samples: LO2, 293T; Cellular Location: basement membrane, extracellular matrix, extracellular space
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.66 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Nidogen in extracts of various cell lines using Nidogen Polyclonal Antibody (Product # PA5-97016) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nidogen in paraffin-embedded human stomach using Nidogen Polyclonal Antibody (Product # PA5-97016) at a dilution of 1:200.
