BAF2570

antibody from R&D Systems

Targeting: NID1 entactin, NID

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

BAF2570

Provider

R&D Systems

Product name

Human Nidogen-1/Entactin Biotinylated Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Nidogen-1/Entactin in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human Nidogen-2 is observed.

Reactivity

Human

Host

Goat

Conjugate

Biotin

Antigen sequence

AAH45606.1


Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Nidogen-1/Entactin in Human Heart. Nidogen-1/Entactin was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human Nidogen-1/Entactin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2570) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.