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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA5-17790 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-PLA2G4A (Ser505) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Concentration
- 250 µg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-PLA2G4A Polyclonal Antibody (Product # PA5-17790) and a ~85 kDa band was observed across the panel tested except IMR-32 and Reh. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), NIH/3T3 (Lane 3), IMR-32 (Lane 4) and Reh (Lane 5) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
