PA5-29100

antibody from Invitrogen Antibodies

Targeting: PLA2G4A cPLA2-alpha, PLA2G4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29100

Provider

Invitrogen Antibodies

Product name

PLA2G4A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Raji, NIH-3T3. Predicted reactivity: Mouse (95%), Rat (95%), Rabbit (92%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.3 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The nucleus acts as a ruler tailoring cell responses to spatial constraints.

Lomakin AJ, Cattin CJ, Cuvelier D, Alraies Z, Molina M, Nader GPF, Srivastava N, Sáez PJ, Garcia-Arcos JM, Zhitnyak IY, Bhargava A, Driscoll MK, Welf ES, Fiolka R, Petrie RJ, De Silva NS, González-Granado JM, Manel N, Lennon-Duménil AM, Müller DJ, Piel M
Science (New York, N.Y.) 2020 Oct 16;370(6514)

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Lipid fingerprint image accurately conveys human colon cell pathophysiologic state: A solid candidate as biomarker.

Bestard-Escalas J, Garate J, Maimó-Barceló A, Fernández R, Lopez DH, Lage S, Reigada R, Khorrami S, Ginard D, Reyes J, Amengual I, Fernández JA, Barceló-Coblijn G
Biochimica et biophysica acta 2016 Dec;1861(12 Pt A):1942-1950

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PLA2G4A using 30 µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PLA2G4A polyclonal antibody (Product # PA5-29100) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PLA2G4A Polyclonal Antibody (Product # PA5-29100). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3 . 7.5% SDS PAGE. PLA2G4A Polyclonal Antibody (Product # PA5-29100) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PLA2G4A Polyclonal Antibody (Product # PA5-29100). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLA2G4A Polyclonal Antibody (Product # PA5-29100) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PLA2G4A in paraformaldehyde-fixed HeLa cells using a PLA2G4A polyclonal antibody (Product # PA5-29100) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of PLA2G4A was performed in paraffin-embedded mouse duodenum tissue using PLA2G4A Polyclonal Antibody (Product # PA5-29100) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of PLA2G4A was performed in paraffin-embedded mouse liver tissue using PLA2G4A Polyclonal Antibody (Product # PA5-29100) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PLA2G4A (Product # PA5-29100) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.