Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- ABIN498079 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) (Internal Region) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide from the internal region of the protein sequence according to NP_077734.1. Genename: PLA2G4A
- Description
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Antigen sequence
C-QEKTFRQQRKEHIR
- Epitope
- Internal Region
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references JNK and ceramide kinase govern the biogenesis of lipid droplets through activation of group IVA phospholipase A2.
Gubern A, Barceló-Torns M, Barneda D, López JM, Masgrau R, Picatoste F, Chalfant CE, Balsinde J, Balboa MA, Claro E
The Journal of biological chemistry 2009 Nov 20;284(47):32359-69
The Journal of biological chemistry 2009 Nov 20;284(47):32359-69
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis: AP20181PU-N PLA2G4A antibody staining of HeLa lysate at 1 μg/ml (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.