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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-46927 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Midkine Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Midkine is observed, and less than 1% cross-reactivity with recombinant human Pleiotrophin is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Midkine in SH‚SY5Y human neuroblastoma cell line. Samples were incubated in Midkine polyclonal antibody (Product # PA5-46927) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Midkine at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Midkine in 0.2 mg/mL lysates of SH‚SY5Y human neuroblastoma cell line. Samples were incubated in Midkine polyclonal antibody (Product # PA5-46927) using a dilution of 100 µg/mL followed by HRP-conjugated Anti-Goat IgG at a dilution of 0.0763888888888889. A specific band was detected for Midkine at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Midkine in immersion fixed paraffin-embedded sections of human prostate. Samples were incubated in Midkine polyclonal antibody (Product # PA5-46927) using a dilution of 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cell cytoplasm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Midkine in immersion fixed paraffin-embedded sections of human ovarian array. Samples were incubated in Midkine polyclonal antibody (Product # PA5-46927) using a dilution of 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
