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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- ELISA [1]
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- Product number
- 500-P171-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Midkine Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Midkine. Anti-Human Midkine-specific antibody was purified by affinity chromatography employing an immobilized Human Midkine matrix. Sandwich ELISA: To detect Human Midkine by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Midkine (500-P171Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Midkine. Western Blot: To detect Human Midkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Midkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 1 mg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references NGPF2 triggers synaptic scaling up through ALK-LIMK-cofilin-mediated mechanisms.
Proangiogenic factor midkine is increased in melanoma patients with sleep apnea and induces tumor cell proliferation.
Midkine is a NF-kappaB-inducible gene that supports prostate cancer cell survival.
Recruitment of insulin receptor substrate-1 and activation of NF-kappaB essential for midkine growth signaling through anaplastic lymphoma kinase.
Zhou Z, He G, Zhang X, Lv X, Zhang X, Liu A, Xia S, Xie H, Dang R, Han L, Qi J, Meng Y, Yu S, Xie W, Jia Z
Cell reports 2021 Aug 17;36(7):109515
Cell reports 2021 Aug 17;36(7):109515
Proangiogenic factor midkine is increased in melanoma patients with sleep apnea and induces tumor cell proliferation.
Cubillos-Zapata C, Martínez-García MÁ, Díaz-García E, Toledano V, Campos-Rodríguez F, Sánchez-de-la-Torre M, Nagore E, Martorell-Calatayud A, Hernández Blasco L, Pastor E, Abad-Capa J, Montserrat JM, Cabriada-Nuño V, Cano-Pumarega I, Corral-Peñafiel J, Arias E, Mediano O, Somoza-González M, Dalmau-Arias J, Almendros I, Farré R, López-Collazo E, Gozal D, García-Río F, Spanish Sleep Network
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Dec;34(12):16179-16190
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Dec;34(12):16179-16190
Midkine is a NF-kappaB-inducible gene that supports prostate cancer cell survival.
You Z, Dong Y, Kong X, Beckett LA, Gandour-Edwards R, Melamed J
BMC medical genomics 2008 Feb 14;1:6
BMC medical genomics 2008 Feb 14;1:6
Recruitment of insulin receptor substrate-1 and activation of NF-kappaB essential for midkine growth signaling through anaplastic lymphoma kinase.
Kuo AH, Stoica GE, Riegel AT, Wellstein A
Oncogene 2007 Feb 8;26(6):859-69
Oncogene 2007 Feb 8;26(6):859-69
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human Midkine by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of Midkine Polyclonal Antibody (Product # 500-P171-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech Midkine Polyclonal Antibody, Biotin (Product # 500-P171BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Midkine.
