PA5-28466

antibody from Invitrogen Antibodies

Targeting: SH3GL2 CNSA2, EEN-B1, SH3D2A, SH3P4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28466

Provider

Invitrogen Antibodies

Product name

SH3GL2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: mouse brain, rat brain. Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (87%), Rabbit (87%), Chicken (93%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SH3GL2 using anti-SH3GL2 Polyclonal Antibody (PA5-28466), in Mouse Brain (20 ug of cell lysate) and at a dilution of 1:10000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SH3GL2 was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a SH3GL2 Polyclonal Antibody (Product # PA5-28466) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SH3GL2 was performed in DIV9 rat E18 primary cortical neurons cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SH3GL2 Polyclonal Antibody (Product # PA5-28466) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.