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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MAB8215 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Caspase-14 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Caspase-14 in direct ELISA and Western Blot.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Caspase-14 by Western Blot. Western blot shows lysates of human skin tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Caspase-14 Monoclonal Antibody (Catalog # MAB8215) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for full length Caspase-14 at approximately 28-30 kDa and the p10 subunit at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Caspase-14 by Simple WesternTM. Simple Western lane view shows lysates of human skin tissue, loaded at 0.5 mg/mL. Specific bands were detected for full length Caspase-14 at approximately 36 kDa and the p10 subunit at approximately 12 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human Caspase-14 Monoclonal Antibody (Catalog # MAB8215). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Caspase-14 in Human Epidermis. Caspase-14 was detected in immersion fixed paraffin-embedded sections of human epidermis using Mouse Anti-Human Caspase-14 Monoclonal Antibody (Catalog # MAB8215) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei of suprabasal keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
