MA5-29082

antibody from Invitrogen Antibodies

Targeting: CASP14 MGC119078, MGC119079, MICE

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29082

Provider

Invitrogen Antibodies

Product name

Caspase 14 Monoclonal Antibody (05)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human Caspase-14/CASP14.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Human Caspase 14 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Caspase 14 Monoclonal Antibody (5) (Product # MA5-29082, 1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®594-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody , counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human CASP14 expression in HeLa cells. The cells were treated, and stained with Caspase 14 Monoclonal Antibody (Product # MA5-29082), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of Human Caspase 14 expression in HeLa cells. The cells were treated according to manufacturer’s manual, and stained with Caspase 14 Monoclonal Antibody (5) (Product # MA5-29082), then a FITC-conjugated Secondary antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.