Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP2-19784 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal PGK1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Yeast
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Hsp90 induces increased genomic instability toward DNA-damaging agents by tuning down RAD53 transcription.
Khurana N, Laskar S, Bhattacharyya MK, Bhattacharyya S
Molecular biology of the cell 2016 Aug 1;27(15):2463-78
Molecular biology of the cell 2016 Aug 1;27(15):2463-78
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: PGK1 Antibody [NBP2-19784] - PGK1 antibody detects PGK1 protein in 1% O2-treated samples by Western blot analysis. Upper panel: PGK1 antibody. Lower panel: beta Actin antibody. A: 30 ug HeLa whole cell lysate/extract (untreated). B: 30 ug HeLa whole cell lysate/extract (1% O2 treatment for 24 hours).
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PGK1 Antibody [NBP2-19784] - PGK1 antibody detects PGK1 protein by Western blot analysis. 50 ug Rat liver lysate/extract, 10% SDS-PAGE, PGK1 antibody dilution: 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PGK1 Antibody [NBP2-19784] - 50 ug mouse brain whole cell lysate, 10% SDS PAGE, antibody at 1:1000.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PGK1 Antibody [NBP2-19784] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted by 1:1000.
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: PGK1 Antibody [NBP2-19784] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PGK1 Antibody [NBP2-19784] - Analysis of paraffin-embedded hepatoma, using antibody at 1:500 dilution.