AP7094b

antibody from Abcepta

Targeting: PGK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7094b

Provider

Abcepta

Proper citation

Abgent Cat#AP7094b, RRID:AB_2161222

Product name

PGK1 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Submitted references

Cigarette smoking exposure alters pebp1 DNA methylation and protein profile involved in MAPK signaling pathway in mice testis.

Xu W, Fang P, Zhu Z, Dai J, Nie D, Chen Z, Qin Q, Wang L, Wang Z, Qiao Z
Biology of reproduction 2013 Dec;89(6):142

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Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice.

Danshina PV, Geyer CB, Dai Q, Goulding EH, Willis WD, Kitto GB, McCarrey JR, Eddy EM, O'Brien DA
Biology of reproduction 2010 Jan;82(1):136-45

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Opposite pathobiochemical fate of pyruvate kinase and adenylate kinase in aged rat skeletal muscle as revealed by proteomic DIGE analysis.

Doran P, O'Connell K, Gannon J, Kavanagh M, Ohlendieck K
Proteomics 2008 Jan;8(2):364-77

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Role of hypoxia inducible factor-1 alpha in modulation of apoptosis resistance.

Kilic M, Kasperczyk H, Fulda S, Debatin KM
Oncogene 2007 Mar 29;26(14):2027-38

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Proteomic profiling reveals a severely perturbed protein expression pattern in aged skeletal muscle.

O'Connell K, Gannon J, Doran P, Ohlendieck K
International journal of molecular medicine 2007 Aug;20(2):145-53

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Parkinson's disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity.

West AB, Moore DJ, Biskup S, Bugayenko A, Smith WW, Ross CA, Dawson VL, Dawson TM
Proceedings of the National Academy of Sciences of the United States of America 2005 Nov 15;102(46):16842-7

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Western blot analysis of lysates from Hela, U-937 cell line (from left to right), using PGK1 Antibody (G132)(Cat. #AP7094b). AP7094b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane."

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of PGK1 Antibody (Center)(Cat#AP7094b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50

Flow cytometry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

PGK1 Antibody (Center) (Cat. #AP7094b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50