AP7169a

antibody from Abcepta

Targeting: PGK1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7169a

Provider

Abcepta

Proper citation

Abgent Cat#AP7169a, RRID:AB_2161230

Product name

PGK1 Antibody (Center S320)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Submitted references

A biotinylated analog of the anti-proliferative prostaglandin A1 allows assessment of PPAR-independent effects and identification of novel cellular targets for covalent modification.

Garzón B, Gayarre J, Gharbi S, Díez-Dacal B, Sánchez-Gómez FJ, Timms JF, Pérez-Sala D
Chemico-biological interactions 2010 Jan 5;183(1):212-21

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Western blot analysis of lysates from A431, Hela, U-937, BA/F3 cell line (from left to right), using PGK1 Antibody (S320) (Cat. #AP7169a). AP7169a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane."

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 Antibody (Center S320)(Cat.#AP7169a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50