Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF5455 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat PGK1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human, mouse and rat PGK1 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P00558
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Metabolic alterations derived from absence of Two-Pore Channel 1 at cardiac level.
Metabolic alterations derived from absence of Two-Pore Channel 1 at cardiac level.
Garcia-Rua V, Feijoo-Bandin S, Garcia-Vence M, Aragon-Herrera A, Bravo SB, Rodriguez-Penas D, Mosquera-Leal A, Lear PV, Parrington J, Alonso J, Rosello-Lleti E, Portoles M, Rivera M, Gonzalez-Juanatey JR, Lago F
Journal of biosciences 2016 Dec;41(4):643-658
Journal of biosciences 2016 Dec;41(4):643-658
Metabolic alterations derived from absence of Two-Pore Channel 1 at cardiac level.
Garcia-Rua V, Feijoo-Bandin S, Garcia-Vence M, Aragon-Herrera A, Bravo SB, Rodriguez-Penas D, Mosquera-Leal A, Lear PV, Parrington J, Alonso J, Rosello-Lleti E, Portoles M, Rivera M, Gonzalez-Juanatey JR, Lago F
Journal of biosciences 2016 Dec;41(4):643-658
Journal of biosciences 2016 Dec;41(4):643-658
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat PGK1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, U937 human histiocytic lymphoma cell line, BaF3 mouse pro-B cell line, A20 mouse B cell lymphoma cell line, Rat-2 rat embryonic fibroblast cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PGK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PGK1 at approximately 43 to 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.