AP33120PU-N

antibody from Acris Antibodies GmbH

Targeting: CSTF2 CstF-64

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

AP33120PU-N

Provider

Acris Antibodies GmbH

Product name

anti CSTF2 (betaCstF-64 variant 1/3)

Antibody type

Polyclonal

Antigen

Peptide with sequence from the internal region of the protein sequence according to ACF05702.1 and ACF05700.1.

Reactivity

Mouse

Host

Goat

Vial size

0.1 mg

Concentration

0.5 mg/ml

Immunoprecipitation

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

CSTF2 Antibody Cat.-No AP33120PU-N (2 µg) used to pull down beta Cstf-64 from Mouse Brain (Lane 5) lysate (500 µg protein) while it cannot pull down Cstf-64 from Mouse Liver (Lane 6) lysate (500 µg) protein) using Protein G-coated magnetic beads. A 1/40th part of the lysates were loaded in Lanes 1 (Brain) and 2 (Liver) before IP and in lanes 3 (Brain) and 4 (Liver) after IP. Western blot probed with Mouse anti-beta Cstf-64/Cstf-64 antibody and detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

HeLa lysate either in RIPA buffer (Lanes 1, 2, 4, 6) or TX100 buffer (Lanes 3, 5, 7) overexpressing Mouse ßCstf-64 (DYKDDDDK tagged) immunoprecipitated using AP33120PU-N (2 µg) with Protein G-coated magnetic beads in lanes 6 and 7 and probed with anti-DYKDDDDK (1/1000). Mock-transfected HeLa in Lane 1. A 1/40th part of the lysate was loaded in Lanes 2 and 3 before IP and in Lanes 4 an 5 after IP. Primary incubations were for 1 hour. Detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA.