Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-C204975 - Provider product page
- Provider
- LSBio
- Product name
- CSTF2 / CstF-64 Antibody (aa309-320) LS-C204975
- Antibody type
- Polyclonal
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Mouse, Porcine
- Host
- Goat
- Storage
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- CSTF2 antibody HeLa lysate either in RIPA buffer (lanes 1, 2, 4, 6) or TX100 buffer (lanes 3, 5, 7) overexpressing Mouse [Character df]Cstf-64 (DYKDDDDK tagged) immunoprecipitated using (2 ug) with Protein G-coated magnetic beads in lanes 6 and 7 and probed with anti-DYKDDDDK (1/1000). Mock-transfected HeLa in Lane 1. A 1/40th part of the lysate was loaded in lanes 2 and 3 before IP and in lanes 4 an 5 after IP. Primary incubations were for 1 hour. Detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- CSTF2 antibody (2 ug) used to pull down [Character df]Cstf-64 from Mouse Brain (lane 5) lysate (500 ug protein) while it cannot pull down Cstf-64 from Mouse Liver (lane 6) lysate (500 ug) protein) using Protein G-coated magnetic beads. A 1/40th part of the lysates were loaded in lanes 1 (Brain) and 2 (Liver) before IP and in lanes 3 (Brain) and 4 (Liver) after IP. Western blot probed with mouse anti-[Character df]Cstf-64/Cstf-64 antibody and detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA.