PA5-37985

antibody from Invitrogen Antibodies

Targeting: CSTF2 CstF-64

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-37985

Provider

Invitrogen Antibodies

Product name

CSTF2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.

Reactivity

Human, Mouse

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of CSTF2 using CSTF2 Polyclonal Antibody (Product # PA5-37985) (2 µg) in Mouse Brain (lane 5) lysate (500 µg protein) while it cannot pull down CSTF2 from Mouse Liver (lane 6) lysate (500 µg protein) using Protein G-coated magnetic beads. A 1/40th part of the lysates were loaded in lanes 1 (Brain) and 2 (Liver) before IP and in lanes 3 (Brain) and 4 (Liver) after IP. Western blot probed with mouse anti-CSTF2 antibody and detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CSTF2 in human placenta. Samples were incubated with CSTF2 polyclonal antibody (Product # PA5-37985).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CSTF2 in Human Tonsil (Formalin-Fixed, Paraffin-Embedded). Samples were incubated with CSTF2 polyclonal antibody (Product # PA5-37985).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation and Western blot analysis of beta CstF-64 variant using 2 µg of polyclonal antibody (Product # PA5-37985) to pull down beta-Cstf-64 from mouse brain lysate (lane 5, 500 µg protein). The antibody did not pull down Cstf-64 from mouse liver (lane 6, 500 µg protein) using Protein G-coated magnetic beads. A 1/40th section of the lysates was loaded onto lane 1 (brain) and lane 2 (liver) before IP, and onto lanes 3 (brain) and 4 (liver) after IP. Western blot was performed using chemiluminescent detection.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of beta CstF-64 variant in HeLa lysate, either in RIPA buffer (lanes 1, 2, 4, 6) or TX100 buffer (lanes 3, 5, 7). Lysates overexpressing mouse beta-Cstf-64 (DYKDDDDK tagged) immunoprecipitated using 2 µg of b-CstF-64 polyclonal antibody (Product # PA5-37985) with Protein G-coated magnetic beads (lanes 6, 7). Samples were probed with a DYKDDDDK antibody at a dilution of 1:1000. Lane 1 shows mock-transfected HeLa cells. A 1/40th section of the lysate was loaded onto lanes 2 and 3 before IP, and in lanes 4 an 5 following IP.