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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NB100-57531 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-57531, RRID:AB_921072
- Product name
- Rabbit Polyclonal Cleavage stimulation factor 2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cleavage stimulation factor 2 Antibody [NB100-57531] - Whole cell lysate (50 ug) from NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CSTF64 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Cleavage stimulation factor 2 Antibody [NB100-57531] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-57531 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. CSTF64 was also immunoprecipitated by rabbit anti-CSTF64 antibody NB100-57530, which recognizes an upstream epitope.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cleavage stimulation factor 2 Antibody [NB100-57531] - Human ovarian carcinoma (left) and mouse teratoma (right). Antibody used at a dilution of 1:1000 (1ug/ml).
