PA5-21782

antibody from Invitrogen Antibodies

Targeting: NAPA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21782

Provider

Invitrogen Antibodies

Product name

SNAP alpha Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2, mouse brain, rat brain.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of alpha SNAP using 50 µg of mouse brain lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an Alpha SNAP polyclonal antibody (Product # PA5-21782) at a dilution of 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SNAP alpha Polyclonal Antibody (Product # PA5-21782). Sample (30 µg whole cell lysate). A: Hep G2. 10% SDS PAGE. SNAP alpha Polyclonal Antibody (Product # PA5-21782) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SNAP alpha was performed by separating 50 µg of various tissue extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a SNAP alpha Polyclonal Antibody (Product # PA5-21782) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SNAP alpha Polyclonal Antibody (Product # PA5-21782). Sample (30 µg whole cell lysate). A: Hep G2. 10% SDS PAGE. SNAP alpha Polyclonal Antibody (Product # PA5-21782) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of alpha SNAP in methanol-fixed A431 cells using an Alpha SNAP polyclonal antibody (Product # PA5-21782) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SNAP alpha was performed in DIV10 rat E18 primary hippocampal neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: SNAP alpha Polyclonal Antibody (Product # PA5 21782) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed A431, using alpha SNAP (Product # PA5-21782) antibody at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SNAP alpha Polyclonal Antibody detects alpha SNAP protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. SNAP alpha Polyclonal Antibody (Product # PA5-21782) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.