GTX104707
antibody from GeneTex
Targeting: STMN1
C1orf215, FLJ32206, Lag, LAP18, OP18, PP17, PP19, PR22, SMN
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX104707 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104707, RRID:AB_1241361
- Product name
- Stathmin 1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.
Proteomic analysis of honokiol-induced cytotoxicity in thyroid cancer cells.
Biomarker discovery for neuroendocrine cervical cancer.
Chang SJ, Liao EC, Yeo HY, Kuo WH, Chen HY, Tsai YT, Wei YS, Chen YJ, Wang YS, Li JM, Shih CC, Chan CH, Lai ZY, Chou HC, Chuang YJ, Chan HL
Archives of biochemistry and biophysics 2018 Jun 1;647:10-32
Archives of biochemistry and biophysics 2018 Jun 1;647:10-32
Proteomic analysis of honokiol-induced cytotoxicity in thyroid cancer cells.
Chou HC, Lu CH, Su YC, Lin LH, Yu HI, Chuang HH, Tsai YT, Liao EC, Wei YS, Yang YT, Chien YA, Yu XR, Lee YR, Chan HL
Life sciences 2018 Aug 15;207:184-204
Life sciences 2018 Aug 15;207:184-204
Biomarker discovery for neuroendocrine cervical cancer.
Lin LH, Chang SJ, Hu RY, Lin MW, Lin ST, Huang SH, Lyu PC, Chou HC, Lai ZY, Chuang YJ, Chan HL
Electrophoresis 2014 Jul;35(14):2039-45
Electrophoresis 2014 Jul;35(14):2039-45
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Stathmin 1 antibody detects STMN1 protein by Western blot analysis.A. 50 ?g mouse brain lysate/extract12 % SDS-PAGEStathmin 1 antibody (GTX104707) dilution: 1:10000
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- GeneTex (provider)
- Main image
- Experimental details
- Stathmin 1 antibody detects STMN1 protein by Western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract B. 30 ?g Rat2 whole cell lysate/extract12 % SDS-PAGEStathmin 1 antibody (GTX104707) dilution: 1:2000
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- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 £gg of whole cell lysate)A:293TB:A431(GTX27909)C:H129912% SDS PAGEGTX104707 diluted at 1:3000
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- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Stathmin 1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody (GTX104707) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Stathmin 1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody (GTX104707) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody (GTX104707) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Stathmin 1 antibody detects Stathmin 1 protein at cytoplasm by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Stathmin 1 protein stained by Stathmin 1 antibody (GTX104707) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Stathmin 1 antibody detects Stathmin 1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Stathmin 1 protein stained by Stathmin 1 antibody (GTX104707) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Stathmin 1 antibody detects Stathmin 1 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Stathmin 1 antibody (GTX104707) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Stathmin 1 antibody detects Stathmin 1 protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: Stathmin 1 protein stained by Stathmin 1 antibody (GTX104707) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).