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LearnABIN256415
antibody from antibodies-online
Targeting: STMN1
C1orf215, FLJ32206, Lag, LAP18, OP18, PP17, PP19, PR22, SMN
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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- Product number
- ABIN256415 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Stathmin 1 (STMN1) antibody
- Antibody type
- Polyclonal
- Antigen
- Modified peptide.
- Description
- Aff - Purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 mL
- Storage
- -20°C
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Hela cells were treated with 300 uM mimosine for 16 hrs, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole (lane 2) for an additional 18hrs and cell extract prepared, resolved by electrophoresis, and transferred to nitrocellulose. Nitrocellulose blots in Panel A were treated overnight with 100 U/ml calf intestinal phosphatase (CIP) and probed with rabbit antibodies reactive against phosphorylated Stathmin/Op18 (Ser16). Nitrocellulose blots that were not treated with CIP (Panel B) were probed with rabbit antibodies reactive against phosphorylated Stathmin/Op18 (Ser16). In both cases proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
