620201

antibody from BioLegend

Targeting: STMN1 C1orf215, FLJ32206, Lag, LAP18, OP18, PP17, PP19, PR22, SMN

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

620201

Provider

BioLegend

Proper citation

BioLegend Cat#620201, RRID:AB_2197103

Product name

Purified anti-Stathmin/Op18 Phospho (Ser16)

Antibody type

Polyclonal

Antigen

Modified peptide

Description

Stathmin/Op18 is a 21 - 23 kD member of the Stathmin family. This protein is localized in the cytoplasm and becomes nuclear during the S/G2 phase of the cell cycle. Stathmin/Op18 acts as a microtubule destabilizer in mitotic spindle regulation and sequesters tubulin dimers into assembly-incompetent complexes. This protein participates as an intracellular relay integrating regulatory signals of the cellular environment and has been shown to be involved in megakaryocyte polyploidization. Stathmin/Op18 can be phosphorylated in response to heat; Ser62 phosphorylation reduces tubulin binding ability. This protein can also be modified by acetylation. Stathmin/Op18 has been shown to interact with tubulin, KIST, CaM kinase II and IV, Cdc2, MAPK, and Cdk1. The Poly6202 antibody recognizes human phosphorylated stathmin/Op18 (Ser16) and has been shown to be useful for Western blotting.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

50 µl

Storage

Upon receipt, store frozen at -20° C.

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Hela cells were treated with 300 µM mimosine for 16 hrs, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole (lane 2) for an additional 18 hrs and cell extract prepared, resolved by electrophoresis, and transferred to nitrocellulose. Nitrocellulose blots were treated overnight with 100 U/ml calf intestinal phosphatase (CIP) (panel A), or not treated with CIP (panel B), and probed with anti-phosphorylated Stathmin/Op18 (Ser16), Poly6202.

Conjugate

Unconjugated