PA5-28092
antibody from Invitrogen Antibodies
Targeting: STMN1
C1orf215, FLJ32206, Lag, LAP18, OP18, PP17, PP19, PR22, SMN
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [3]
- Immunohistochemistry [3]
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- Product number
- PA5-28092 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Stathmin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLa, 293T, A431, H1299, mouse brain, rat brain, PC-12, Rat2. Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Stathmin 1 was performed by separating 50 µg of various tissue extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a Stathmin 1 Polyclonal Antibody (Product # PA5-28092) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Stathmin 1 Polyclonal Antibody (Product # PA5-28092). Sample (30 µg of whole cell lysate). A: 293T. B: A431. C: H1299. 12% SDS PAGE. Stathmin 1 Polyclonal Antibody (Product # PA5-28092) diluted at 1:3,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Stathmin 1 Polyclonal Antibody detects STMN1 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract. B. 30 µg Rat2 whole cell lysate/extract.12 % SDS-PAGE. Stathmin 1 Polyclonal Antibody (Product # PA5-28092) dilution: 1:2,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Stathmin 1 Polyclonal Antibody detects STMN1 protein by Western blot analysis. A. 50 µg mouse brain lysate/extract.12 % SDS-PAGE. Stathmin 1 Polyclonal Antibody (Product # PA5-28092) dilution: 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Stathmin1 was achieved by transfecting HeLa with Stathmin1 specific siRNAs (Silencer® select Product # s8093). Western blot analysis (Fig. a) was performed using whole cell extracts from the Stathmin 1 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Stathmin 1 Polyclonal Antibody (Product # PA5-28092, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of specific signal upon siRNA mediated knock down confirms that antibody is specific to Stathmin1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Stathmin 1 Polyclonal Antibody (Product # PA5-28092) and a 17kDa band corresponding to Stathmin 1 was observed in all the tested cell lines, except A549 and Caco-2. Additional band at ~90kDa was also found across the cell lines. Whole cell lysate (30ug lysate) of HeLa (Lane 1), PC-3 (Lane 2), MDA-MB-231 (Lane 3), T-47D (Lane 4), A-431 (Lane 5), Caco-2 (Lane 6), A549 (Lane 7) and U-2 OS (Lane 8) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Stathmin 1 was performed in SK N SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Stathmin 1 Polyclonal Antibody (Product # PA5 28092) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Stathmin 1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Stathmin 1 Polyclonal Antibody (Product # PA5 28092) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Stathmin 1 was performed in SK N SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Stathmin 1 Polyclonal Antibody (Product # PA5 28092) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of Stathmin-1 was performed in frozen-sectioned adult mouse cerebellum tissue using Stathmin 1 Polyclonal Antibody (Product # PA5-28092) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of Stathmin-1 was performed in frozen-sectioned adult mouse cerebellum tissue using Stathmin 1 Polyclonal Antibody (Product # PA5-28092) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Stathmin 1 Polyclonal Antibody detects Stathmin 1 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Stathmin 1 Polyclonal Antibody (Product # PA5-28092) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.