- PA5-28092 - Invitrogen Antibodies STMN1 antibody

No submitted references: Submit reference

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Stathmin 1 was performed by separating 50 µg of various tissue extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a Stathmin 1 Polyclonal Antibody (Product # PA5-28092) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Stathmin 1 Polyclonal Antibody (Product # PA5-28092). Sample (30 µg of whole cell lysate). A: 293T. B: A431. C: H1299. 12% SDS PAGE. Stathmin 1 Polyclonal Antibody (Product # PA5-28092) diluted at 1:3,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Stathmin 1 Polyclonal Antibody detects STMN1 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract. B. 30 µg Rat2 whole cell lysate/extract.12 % SDS-PAGE. Stathmin 1 Polyclonal Antibody (Product # PA5-28092) dilution: 1:2,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Stathmin 1 Polyclonal Antibody detects STMN1 protein by Western blot analysis. A. 50 µg mouse brain lysate/extract.12 % SDS-PAGE. Stathmin 1 Polyclonal Antibody (Product # PA5-28092) dilution: 1:10,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Stathmin1 was achieved by transfecting HeLa with Stathmin1 specific siRNAs (Silencer® select Product # s8093). Western blot analysis (Fig. a) was performed using whole cell extracts from the Stathmin 1 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Stathmin 1 Polyclonal Antibody (Product # PA5-28092, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of specific signal upon siRNA mediated knock down confirms that antibody is specific to Stathmin1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Stathmin 1 Polyclonal Antibody (Product # PA5-28092) and a 17kDa band corresponding to Stathmin 1 was observed in all the tested cell lines, except A549 and Caco-2. Additional band at ~90kDa was also found across the cell lines. Whole cell lysate (30ug lysate) of HeLa (Lane 1), PC-3 (Lane 2), MDA-MB-231 (Lane 3), T-47D (Lane 4), A-431 (Lane 5), Caco-2 (Lane 6), A549 (Lane 7) and U-2 OS (Lane 8) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of Stathmin 1 was performed in SK N SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Stathmin 1 Polyclonal Antibody (Product # PA5 28092) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of Stathmin 1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Stathmin 1 Polyclonal Antibody (Product # PA5 28092) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of Stathmin 1 was performed in SK N SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Stathmin 1 Polyclonal Antibody (Product # PA5 28092) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Frozen) analysis of Stathmin-1 was performed in frozen-sectioned adult mouse cerebellum tissue using Stathmin 1 Polyclonal Antibody (Product # PA5-28092) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Frozen) analysis of Stathmin-1 was performed in frozen-sectioned adult mouse cerebellum tissue using Stathmin 1 Polyclonal Antibody (Product # PA5-28092) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Stathmin 1 Polyclonal Antibody detects Stathmin 1 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Stathmin 1 Polyclonal Antibody (Product # PA5-28092) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PA5-28092