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- Antibody Data
- Antigen structure
- References [19]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- 13509-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#13509-1-AP, RRID:AB_2257094
- Product name
- p115, USO1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated p115, USO1 antibody (Cat. #13509-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Golgi organization is a determinant of stem cell function in the small intestine.
A pref-1-controlled non-inflammatory mechanism of insulin resistance.
The Immune-Specific E3 Ubiquitin Ligase MARCH1 Is Upregulated during Human Cytomegalovirus Infection to Regulate Iron Levels.
Sec22b Regulates Inflammatory Responses by Controlling the Nuclear Translocation of NF-κB and the Secretion of Inflammatory Mediators.
Focused CRISPR-Cas9 genetic screening reveals USO1 as a vulnerability in B-cell acute lymphoblastic leukemia.
Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.
Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.
An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.
Herpes Simplex Virus 1-Induced Blood-Brain Barrier Damage Involves Apoptosis Associated With GM130-Mediated Golgi Stress.
Defining the proteolytic landscape during enterovirus infection.
Human Cytomegalovirus Decreases Major Histocompatibility Complex Class II by Regulating Class II Transactivator Transcript Levels in a Myeloid Cell Line.
Inhibition of diverse opportunistic viruses by structurally optimized retrograde trafficking inhibitors.
GM130 and p115 play a key role in the organisation of the early secretory pathway during skeletal muscle differentiation.
Small GTPase Rab1B is associated with ATG9A vesicles and regulates autophagosome formation.
Globozoospermia and lack of acrosome formation in GM130-deficient mice.
cTAGE5 deletion in pancreatic β cells impairs proinsulin trafficking and insulin biogenesis in mice.
The Arf GEF GBF1 and Arf4 synergize with the sensory receptor cargo, rhodopsin, to regulate ciliary membrane trafficking.
AmotL2 disrupts apical-basal cell polarity and promotes tumour invasion.
Golgi disruption and early embryonic lethality in mice lacking USO1.
Scharaw S, Sola-Carvajal A, Belevich I, Webb AT, Das S, Andersson S, Pentinmikko N, Villablanca EJ, Goldenring JR, Jokitalo E, Coffey RJ, Katajisto P
bioRxiv : the preprint server for biology 2023 Mar 24;
bioRxiv : the preprint server for biology 2023 Mar 24;
A pref-1-controlled non-inflammatory mechanism of insulin resistance.
Huang Y, Cui D, Chen L, Tong H, Wu H, Muller GK, Qi Y, Wang S, Xu J, Gao X, Fifield KE, Wang L, Xia Z, Vanderluit JL, Liu S, Leng L, Sun G, McGuire J, Young LH, Bucala R, Qi D
iScience 2023 Jun 16;26(6):106923
iScience 2023 Jun 16;26(6):106923
The Immune-Specific E3 Ubiquitin Ligase MARCH1 Is Upregulated during Human Cytomegalovirus Infection to Regulate Iron Levels.
Martin M, Sandhu P, Kumar R, Buchkovich NJ
Journal of virology 2022 Mar 23;96(6):e0180621
Journal of virology 2022 Mar 23;96(6):e0180621
Sec22b Regulates Inflammatory Responses by Controlling the Nuclear Translocation of NF-κB and the Secretion of Inflammatory Mediators.
Arango Duque G, Dion R, Matte C, Fabié A, Descoteaux J, Stäger S, Descoteaux A
Journal of immunology (Baltimore, Md. : 1950) 2021 Nov 1;207(9):2297-2309
Journal of immunology (Baltimore, Md. : 1950) 2021 Nov 1;207(9):2297-2309
Focused CRISPR-Cas9 genetic screening reveals USO1 as a vulnerability in B-cell acute lymphoblastic leukemia.
Jaiswal AK, Truong H, Tran TM, Lin TL, Casero D, Alberti MO, Rao DS
Scientific reports 2021 Jun 23;11(1):13158
Scientific reports 2021 Jun 23;11(1):13158
Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.
Zhang Y, Seemann J
The Journal of cell biology 2021 Jan 4;220(1)
The Journal of cell biology 2021 Jan 4;220(1)
Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.
Yue X, Tiwari N, Zhu L, Ngo HDT, Lim JM, Gim B, Jing S, Wang Y, Qian Y, Lee I
Communications biology 2021 Dec 7;4(1):1370
Communications biology 2021 Dec 7;4(1):1370
An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.
Nüchel J, Tauber M, Nolte JL, Mörgelin M, Türk C, Eckes B, Demetriades C, Plomann M
Molecular cell 2021 Aug 19;81(16):3275-3293.e12
Molecular cell 2021 Aug 19;81(16):3275-3293.e12
Herpes Simplex Virus 1-Induced Blood-Brain Barrier Damage Involves Apoptosis Associated With GM130-Mediated Golgi Stress.
He Q, Liu H, Huang C, Wang R, Luo M, Lu W
Frontiers in molecular neuroscience 2020;13:2
Frontiers in molecular neuroscience 2020;13:2
Defining the proteolytic landscape during enterovirus infection.
Saeed M, Kapell S, Hertz NT, Wu X, Bell K, Ashbrook AW, Mark MT, Zebroski HA, Neal ML, Flodström-Tullberg M, MacDonald MR, Aitchison JD, Molina H, Rice CM
PLoS pathogens 2020 Sep;16(9):e1008927
PLoS pathogens 2020 Sep;16(9):e1008927
Human Cytomegalovirus Decreases Major Histocompatibility Complex Class II by Regulating Class II Transactivator Transcript Levels in a Myeloid Cell Line.
Sandhu PK, Buchkovich NJ
Journal of virology 2020 Mar 17;94(7)
Journal of virology 2020 Mar 17;94(7)
Inhibition of diverse opportunistic viruses by structurally optimized retrograde trafficking inhibitors.
Desai D, Lauver M, Ostman A, Cruz L, Ferguson K, Jin G, Roper B, Brosius D, Lukacher A, Amin S, Buchkovich N
Bioorganic & medicinal chemistry 2019 May 1;27(9):1795-1803
Bioorganic & medicinal chemistry 2019 May 1;27(9):1795-1803
GM130 and p115 play a key role in the organisation of the early secretory pathway during skeletal muscle differentiation.
Giacomello E, Ronchi P, Pepperkok R
Journal of cell science 2019 Jan 23;132(2)
Journal of cell science 2019 Jan 23;132(2)
Small GTPase Rab1B is associated with ATG9A vesicles and regulates autophagosome formation.
Kakuta S, Yamaguchi J, Suzuki C, Sasaki M, Kazuno S, Uchiyama Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Sep;31(9):3757-3773
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Sep;31(9):3757-3773
Globozoospermia and lack of acrosome formation in GM130-deficient mice.
Han F, Liu C, Zhang L, Chen M, Zhou Y, Qin Y, Wang Y, Chen M, Duo S, Cui X, Bao S, Gao F
Cell death & disease 2017 Jan 5;8(1):e2532
Cell death & disease 2017 Jan 5;8(1):e2532
cTAGE5 deletion in pancreatic β cells impairs proinsulin trafficking and insulin biogenesis in mice.
Fan J, Wang Y, Liu L, Zhang H, Zhang F, Shi L, Yu M, Gao F, Xu Z
The Journal of cell biology 2017 Dec 4;216(12):4153-4164
The Journal of cell biology 2017 Dec 4;216(12):4153-4164
The Arf GEF GBF1 and Arf4 synergize with the sensory receptor cargo, rhodopsin, to regulate ciliary membrane trafficking.
Wang J, Fresquez T, Kandachar V, Deretic D
Journal of cell science 2017 Dec 1;130(23):3975-3987
Journal of cell science 2017 Dec 1;130(23):3975-3987
AmotL2 disrupts apical-basal cell polarity and promotes tumour invasion.
Mojallal M, Zheng Y, Hultin S, Audebert S, van Harn T, Johnsson P, Lenander C, Fritz N, Mieth C, Corcoran M, Lembo F, Hallström M, Hartman J, Mazure NM, Weide T, Grandér D, Borg JP, Uhlén P, Holmgren L
Nature communications 2014 Aug 1;5:4557
Nature communications 2014 Aug 1;5:4557
Golgi disruption and early embryonic lethality in mice lacking USO1.
Kim S, Hill A, Warman ML, Smits P
PloS one 2012;7(11):e50530
PloS one 2012;7(11):e50530
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 13509-1-AP( p115, USO1 Antibody) at dilution of 1:1500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using USO1 antibody 13509-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-p115, USO1 (IP:13509-1-AP, 3ug; Detection:13509-1-AP 1:1500) with mouse brain tissue lysate 7000ug.
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 13509-1-AP(p115, USO1 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 13509-1-AP(p115, USO1 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
