Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [4]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 13509-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p115/USO1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.19 mg/mL
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 13509-1-AP ( p115, USO1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13509-1-AP (p115, USO1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13509-1-AP (p115, USO1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (Product # 13509-1-AP) at dilution of 1:1,000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of Hela cells, using USO1 antibody 13509-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13509-1-AP ( p115, USO1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13509-1-AP ( p115, USO1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- IP result of anti-p115, USO1 (IP:13509-1-AP, 3ug; Detection:13509-1-AP 1:1500) with mouse brain tissue lysate 7000ug.