Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-30281 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VDP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, mouse liver. Predicted reactivity: Human (99%), Mouse (94%), Rat (93%), Chicken (88%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Autophagy Inhibition Induces the Secretion of Macrophage Migration Inhibitory Factor (MIF) with Autocrine and Paracrine Effects on the Promotion of Malignancy in Breast Cancer.
Cotzomi-Ortega I, Rosas-Cruz A, Ramírez-Ramírez D, Reyes-Leyva J, Rodriguez-Sosa M, Aguilar-Alonso P, Maycotte P
Biology 2020 Jan 18;9(1)
Biology 2020 Jan 18;9(1)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VDP/p115 using A) 30 µg 293T whole cell extract (B) 30 µg A431 whole cell extract (C) 30 µg HeLa whole cell extract (D) 30 µg HepG2 whole cell extract and E) 30 µg NIH-3T3 whole cell extract. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a VDP/p115 polyclonal antibody (Product # PA5-30281) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using VDP Polyclonal Antibody (Product # PA5-30281). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with VDP/p115 antibody [N1N2], N-term VDP Polyclonal Antibody (Product # PA5-30281) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using VDP Polyclonal Antibody (Product # PA5-30281). Sample (50 µg of whole cell lysate). Lane A: Mouse liver . 5% SDS PAGE. VDP Polyclonal Antibody (Product # PA5-30281) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- VDP Polyclonal Antibody [N1N2], N-term detects VDP / p115 protein at cytoplasm and golgi apparatus by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: VDP / p115 stained by VDP Polyclonal Antibody [N1N2], N-term (Product # PA5-30281) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- VDP Polyclonal Antibody [N1N2], N-term detects VDP / p115 protein at cytoplasm and golgi apparatus by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: VDP / p115 stained by VDP Polyclonal Antibody [N1N2], N-term (Product # PA5-30281) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using VDP/p115 (Product # PA5-30281) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 MIF secretion induced by the inhibition of autophagy was mediated by reactive oxygen species (ROS) production, occurred with changes in Uso1/p115 protein levels, and mediated cell survival in the 66cl4 cell line. ROS levels were evaluated by DHE staining by flow cytometry at 16 h of treatment at the indicated concentrations of CQ ( A ). Uso1/p115 protein levels were evaluated by Western blot in the three cell lines studied ( B ). N-acetyl cysteine (NAC) treatment at the indicated concentrations reduced the ROS levels induced by CQ treatment and reduced MIF secretion in the 66cl4 cell line ( C ) both at 16 h of treatment. ISO-1, a MIF inhibitor, increased cell death in the 66cl4 cell line when used in combination with CQ treatment for 24 h ( D , E ). The scale bar in the pictures in ( E ) represents 200 um. Results show mean +/- standard error of three-four independent experiments, * p < 0.05, ** p < 0.01.