R32729

antibody from NSJ Bioreagents

Targeting: TFPI2 PP5, REF1, TFPI-2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R32729

Provider

NSJ Bioreagents

Product name

TFPI2 Antibody

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Concentration

0.5mg/ml

Storage

After reconstitution, the TFPI2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) mouse spleen, 2) human HeLa, 3) human placenta and 4) human MCF7 lysate with TFPI2 antibody at 0.5ug/ml. Expected molecular weight: ~27 kDa (unmodified), 30-35 kDa (glycosylated).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human placental tissue with TFPI2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human A549 cells with TFPI2 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TFPI2 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human U-87 MG cells with TFPI2 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TFPI2 antibody.