LS-C172820

antibody from LSBio

Targeting: BECN1 ATG6, VPS30

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C172820

Provider

LSBio

Product name

BECN1 / Beclin-1 Antibody (clone OTI1F1) LS-C172820

Antibody type

Monoclonal

Description

Protein A/G purified

Reactivity

Human, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

OTI1F1

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BECN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BECN1.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of extracts (35 ug) from 9 different cell lines by using anti-BECN1 monoclonal antibody.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Human liver tissue using anti-BECN1 mouse monoclonal antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Human liver tissue using anti-BECN1 mouse monoclonal antibody.