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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP1818b - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP1818b, RRID:AB_2064335
- Product name
- Beclin 1 Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Curcumin-loaded γ-cyclodextrin liposomal nanoparticles as delivery vehicles for osteosarcoma.
A non-canonical MEK/ERK signaling pathway regulates autophagy via regulating Beclin 1.
Dhule SS, Penfornis P, Frazier T, Walker R, Feldman J, Tan G, He J, Alb A, John V, Pochampally R
Nanomedicine : nanotechnology, biology, and medicine 2012 May;8(4):440-51
Nanomedicine : nanotechnology, biology, and medicine 2012 May;8(4):440-51
A non-canonical MEK/ERK signaling pathway regulates autophagy via regulating Beclin 1.
Wang J, Whiteman MW, Lian H, Wang G, Singh A, Huang D, Denmark T
The Journal of biological chemistry 2009 Aug 7;284(32):21412-24
The Journal of biological chemistry 2009 Aug 7;284(32):21412-24
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "The anti-BECN1 Pab (Cat. #AP1818b) is used in Western blot to detect BECN1 in, from left to right, A2058, Hela, mouse brain, Y79, and HL60 tissue lysates. BECN1(arrow) was detected using the purified Pab."
- Primary Ab dilution
- 1:1000
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of anti-hBECN1-E225 Pab (Cat. #AP1818b) in 293 cell line lysates transiently transfected with the BECN1 gene (2ug/lane). hBECN1-E225(arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Y79 cells using Beclin 1 (BECN1/APG6) Antibody (E225) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
